1aus

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[[Image:1aus.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_1aus| PDB=1aus | SCENE= }}
{{STRUCTURE_1aus| PDB=1aus | SCENE= }}
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'''ACTIVATED UNLIGANDED SPINACH RUBISCO'''
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===ACTIVATED UNLIGANDED SPINACH RUBISCO===
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==Overview==
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The crystal structure of an activated complex of ribulose-1,5-bisphosphate carboxylase/oxygenase from spinach and its product 3-phosphoglycerate has been determined to 2.2 A resolution. The structure is of the open form with the active site accessible to the solvent as observed in the structures of the activated ligand-free enzyme and the complex of the activated enzyme with the substrate ribulose-1,5-bisphosphate. Two molecules of 3-phosphoglycerate are bound per active site. The phosphates of both molecules bind approximately at the same position as the phosphates of ribulose-1,5-bisphosphate or the six-carbon intermediate analogue 2-carboxyarabinitol-1,5-bisphosphate, but one product molecule is swung out from the active site with its carboxylate group pointing toward solution. The present structure points to direct participation of the active site side chain of lysine 175 in later stages of catalysis. This possibility is discussed in the light of mutagenesis studies.
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The line below this paragraph, {{ABSTRACT_PUBMED_9092835}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 9092835 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9092835}}
==About this Structure==
==About this Structure==
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[[Category: Andersson, I.]]
[[Category: Andersson, I.]]
[[Category: Taylor, T C.]]
[[Category: Taylor, T C.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:43:06 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 17:38:06 2008''

Revision as of 14:38, 30 June 2008

Template:STRUCTURE 1aus

ACTIVATED UNLIGANDED SPINACH RUBISCO

Template:ABSTRACT PUBMED 9092835

About this Structure

1AUS is a Protein complex structure of sequences from Spinacia oleracea. Full crystallographic information is available from OCA.

Reference

Structure of a product complex of spinach ribulose-1,5-bisphosphate carboxylase/oxygenase., Taylor TC, Andersson I, Biochemistry. 1997 Apr 1;36(13):4041-6. PMID:9092835

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