1e0b
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(New page: 200px<br /><applet load="1e0b" size="450" color="white" frame="true" align="right" spinBox="true" caption="1e0b, resolution 1.90Å" /> '''CHROMO SHADOW DOMAIN...)
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Revision as of 11:35, 20 November 2007
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CHROMO SHADOW DOMAIN FROM FISSION YEAST SWI6 PROTEIN.
Overview
BACKGROUND: Proteins such as HP1, found in fruit flies and mammals, and, Swi6, its fission yeast homologue, carry a chromodomain (CD) and a chromo, shadow domain (CSD). These proteins are required to form functional, transcriptionally silent centromeric chromatin, and their mutation leads, to chromosome segregation defects. CSDs have only been found in tandem in, proteins containing the related CD. Most HP1-interacting proteins have, been found to associate through the CSD and many of these ligands contain, a conserved pentapeptide motif. RESULTS: The 1.9 A crystal structure of, the Swi6 CSD is presented here. This reveals a novel dimeric structure, that is distinct from the previously reported monomeric nuclear magnetic, resonance (NMR) structure of the CD from the mouse modifier 1 protein, (MoMOD1, also known as HP1beta or M31). A prominent pit with a non-polar, base is generated at the dimer interface, and is commensurate with binding, an extended pentapeptide motif. Sequence alignments based on this, structure highlight differences between CDs and CSDs that are superimposed, on a common structural core. The analyses also revealed a previously, unrecognised circumferential hydrophobic sash around the surface of the CD, structure. CONCLUSIONS: Dimerisation through the CSD of HP1-like proteins, results in the simultaneous formation of a putative protein-protein, interaction pit, providing a potential means of targeting CSD-containing, proteins to particular chromatin sites.
About this Structure
1E0B is a Single protein structure of sequence from Schizosaccharomyces pombe with 1PG as ligand. Full crystallographic information is available from OCA.
Reference
Dimerisation of a chromo shadow domain and distinctions from the chromodomain as revealed by structural analysis., Cowieson NP, Partridge JF, Allshire RC, McLaughlin PJ, Curr Biol. 2000 May 4;10(9):517-25. PMID:10801440
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