1axd

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{{STRUCTURE_1axd| PDB=1axd | SCENE= }}
{{STRUCTURE_1axd| PDB=1axd | SCENE= }}
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'''STRUCTURE OF GLUTATHIONE S-TRANSFERASE-I BOUND WITH THE LIGAND LACTOYLGLUTATHIONE'''
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===STRUCTURE OF GLUTATHIONE S-TRANSFERASE-I BOUND WITH THE LIGAND LACTOYLGLUTATHIONE===
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==Overview==
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Glutathione S-transferases (GSTs) -I and -III are involved in herbicide metabolism in maize and have been intensively studied. Starting with plant tissue from Zea mays var. mutin recombinant GST-I was prepared by heterologous expression in Escherichia coli. The enzyme was crystallized in the presence of lactoylglutathione, a ligand formerly never observed in a GST structure and known as an intermediate of the pharmacologically relevant glyoxalase system. The crystal structure of GST-I has been determined at 2.5 A resolution and exhibits the GST-typical dimer of two identical subunits, each consisting of 214 residues. Compared with other plant GSTs the three-dimensional structure of GST-I primarily shows structural differences in the hydrophobic substrate binding site, the linker segment and the C-terminal region. Furthermore, a comparison of the ligand-bound GST-I structure with the apo structure of GST-III indicates the movement of a ten-residue loop upon binding of the ligand to the active site. This is the first structure-based evidence for an induced fit mechanism of glutathione S-transferases, which has previously been postulated for class pi enzymes. Together with GST-III, GST-I may explain herbicide resistance and selectivity in maize as well as in other agronomic relevant crops.
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(as it appears on PubMed at http://www.pubmed.gov), where 9417926 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9417926}}
==About this Structure==
==About this Structure==
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[[Category: Herbicide detoxification]]
[[Category: Herbicide detoxification]]
[[Category: Transferase]]
[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:48:31 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 17:47:42 2008''

Revision as of 14:47, 30 June 2008

Image:1axd.png

Template:STRUCTURE 1axd

STRUCTURE OF GLUTATHIONE S-TRANSFERASE-I BOUND WITH THE LIGAND LACTOYLGLUTATHIONE

Template:ABSTRACT PUBMED 9417926

About this Structure

1AXD is a Single protein structure of sequence from Zea mays. Full crystallographic information is available from OCA.

Reference

Crystal structure of herbicide-detoxifying maize glutathione S-transferase-I in complex with lactoylglutathione: evidence for an induced-fit mechanism., Neuefeind T, Huber R, Dasenbrock H, Prade L, Bieseler B, J Mol Biol. 1997 Dec 12;274(4):446-53. PMID:9417926

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