1e2u

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(New page: 200px<br /><applet load="1e2u" size="450" color="white" frame="true" align="right" spinBox="true" caption="1e2u, resolution 1.6&Aring;" /> '''LOW TEMPERATURE STUCT...)
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Revision as of 11:38, 20 November 2007

Image:1e2u.gif

1e2u, resolution 1.6Å

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LOW TEMPERATURE STUCTURE OF HYBRID CLUSTER PROTEIN FROM DESULFOVIBRIO VULGARIS TO 1.6A

Overview

The three-dimensional structure of the hybrid cluster protein from, Desulfovibrio vulgaris (Hildenborough) has been determined at 1.6 A, resolution using synchrotron X-ray radiation. The protein can be divided, into three domains: an N-terminal mainly alpha-helical domain and two, similar domains comprising a central beta-sheet flanked by alpha-helices., The protein contains two 4Fe clusters with an edge-to-edge distance of, 10.9 A. Four cysteine residues at the N-terminus of the protein are, ligands to the iron atoms of a conventional [4Fe-4S] cubane cluster. The, second cluster has an unusual asymmetric structure and has been named the, hybrid cluster to reflect the variety of protein ligands, namely two, mu-sulfido bridges, two mu(2)-oxo bridges, and a further disordered, bridging ligand. Anomalous differences in data collected at 1.488 A and, close to the iron edge at 1.743 A have been used to confirm the identity, of the metal and sulfur atoms. The hybrid cluster is buried in the center, of the protein, but is accessible through a large hydrophobic cavity that, runs the length of domain 3. Hydrophobic channels have previously been, identified as access routes to the active centers in redox enzymes with, gaseous substrates. The hybrid cluster is also accessible by a hydrophilic, channel. The [4Fe-4S] cubane cluster is close to an indentation on the, surface of the protein and can also be approached on the opposite side by, a long solvent channel. At the present time, neither the significance of, these channels nor, indeed, the function of the hybrid cluster protein is, known.

About this Structure

1E2U is a Single protein structure of sequence from Desulfovibrio vulgaris with SF4 and FSO as ligands. Full crystallographic information is available from OCA.

Reference

Hybrid-cluster protein (HCP) from Desulfovibrio vulgaris (Hildenborough) at 1.6 A resolution., Cooper SJ, Garner CD, Hagen WR, Lindley PF, Bailey S, Biochemistry. 2000 Dec 12;39(49):15044-54. PMID:11106482

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