1b1u

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{{Seed}}
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{{STRUCTURE_1b1u| PDB=1b1u | SCENE= }}
{{STRUCTURE_1b1u| PDB=1b1u | SCENE= }}
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'''CRYSTAL STRUCTURE OF THE BIFUNCTIONAL INHIBITOR RAGI'''
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===CRYSTAL STRUCTURE OF THE BIFUNCTIONAL INHIBITOR RAGI===
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==Overview==
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The crystal structure of a bifunctional inhibitor of alpha-amylase and trypsin (RATI) from ragi seeds (Indian finger millet, Eleusine coracana Gaertneri) has been determined by X-ray diffraction at 2.2 A resolution. The inhibitor consists of 122 amino acids, with five disulfide bridges, and belongs to the plant alpha-amylase/trypsin inhibitor family. The crystals were grown by the microdialysis method using ammonium sulfate as a precipitating agent. The structure was determined by the molecular-replacement method using as models the structures of Corn Hageman factor inhibitor (CHFI) and of RATI at 2.9 A resolution determined previously. It has been refined to an R factor of 21.9%. The structure shows an r.m.s. deviation for C(alpha) atoms of 2.0 A compared with its own NMR structure, whereas the corresponding value compared with CHFI is found to be 1.4 A. The r.m.s. difference for C(alpha) atoms when compared with the same protein in the structure of the complex with alpha-amylase is 0.7 A. The conformations of trypsin-binding loop and the alpha-amylase-binding N-terminal region were also found to be similar in the crystal structures of native RATI and its complex with alpha-amylase. These regions differed considerably in the NMR structure.
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The line below this paragraph, {{ABSTRACT_PUBMED_10713515}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 10713515 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10713515}}
==About this Structure==
==About this Structure==
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[[Category: Bifunctional]]
[[Category: Bifunctional]]
[[Category: Hydrolase inhibitor]]
[[Category: Hydrolase inhibitor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:57:29 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 18:02:54 2008''

Revision as of 15:02, 30 June 2008

Image:1b1u.png

Template:STRUCTURE 1b1u

CRYSTAL STRUCTURE OF THE BIFUNCTIONAL INHIBITOR RAGI

Template:ABSTRACT PUBMED 10713515

About this Structure

1B1U is a Single protein structure of sequence from Eleusine coracana. This structure supersedes the now removed PDB entry 1jfo. Full crystallographic information is available from OCA.

Reference

Structure of the bifunctional inhibitor of trypsin and alpha-amylase from ragi seeds at 2.2 A resolution., Gourinath S, Alam N, Srinivasan A, Betzel C, Singh TP, Acta Crystallogr D Biol Crystallogr. 2000 Mar;56(Pt 3):287-93. PMID:10713515

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