This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1e2x
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /><applet load="1e2x" size="450" color="white" frame="true" align="right" spinBox="true" caption="1e2x, resolution 2.00Å" /> '''FADR, FATTY ACID RES...)
Next diff →
Revision as of 11:38, 20 November 2007
|
FADR, FATTY ACID RESPONSIVE TRANSCRIPTION FACTOR FROM E. COLI
Overview
FadR is a dimeric acyl coenzyme A (acyl CoA)-binding protein and, transcription factor that regulates the expression of genes encoding fatty, acid biosynthetic and degrading enzymes in Escherichia coli. Here, the 2.0, A crystal structure of full-length FadR is described, determined using, multi-wavelength anomalous dispersion. The structure reveals a dimer and a, two-domain fold, with DNA-binding and acyl-CoA-binding sites located in an, N-terminal and C-terminal domain, respectively. The N-terminal domain, contains a winged helix-turn-helix prokaryotic DNA-binding fold., Comparison with known structures and analysis of mutagenesis data, delineated the site of interaction with DNA. The C-terminal domain has a, novel fold, consisting of a seven-helical bundle with a crossover, topology. Careful analysis of the structure, together with mutational and, biophysical data, revealed a putative hydrophobic acyl-CoA-binding site, buried in the core of the seven-helical bundle. This structure aids in, understanding FadR function at a molecular level, provides the first, structural scaffold for the large GntR family of transcription factors, which are keys in the control of metabolism in bacterial pathogens, and, could thus be a possible target for novel chemotherapeutic agents.
About this Structure
1E2X is a Single protein structure of sequence from Escherichia coli with SO4 as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of FadR, a fatty acid-responsive transcription factor with a novel acyl coenzyme A-binding fold., van Aalten DM, DiRusso CC, Knudsen J, Wierenga RK, EMBO J. 2000 Oct 2;19(19):5167-77. PMID:11013219
Page seeded by OCA on Tue Nov 20 13:45:17 2007
