1b22

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1b22.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1b22.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1b22| PDB=1b22 | SCENE= }}
{{STRUCTURE_1b22| PDB=1b22 | SCENE= }}
-
'''RAD51 (N-TERMINAL DOMAIN)'''
+
===RAD51 (N-TERMINAL DOMAIN)===
-
==Overview==
+
<!--
-
Human Rad51 protein (HsRad51) is a homolog of Escherichia coli RecA protein, and functions in DNA repair and recombination. In higher eukaryotes, Rad51 protein is essential for cell viability. The N-terminal region of HsRad51 is highly conserved among eukaryotic Rad51 proteins but is absent from RecA, suggesting a Rad51-specific function for this region. Here, we have determined the structure of the N-terminal part of HsRad51 by NMR spectroscopy. The N-terminal region forms a compact domain consisting of five short helices, which shares structural similarity with a domain of endonuclease III, a DNA repair enzyme of E. coli. NMR experiments did not support the involvement of the N-terminal domain in HsRad51-HsBrca2 interaction or the self-association of HsRad51 as proposed by previous studies. However, NMR tiration experiments demonstrated a physical interaction of the domain with DNA, and allowed mapping of the DNA binding surface. Mutation analysis showed that the DNA binding surface is essential for double-stranded and single-stranded DNA binding of HsRad51. Our results suggest the presence of a DNA binding site on the outside surface of the HsRad51 filament and provide a possible explanation for the regulation of DNA binding by phosphorylation within the N-terminal domain.
+
The line below this paragraph, {{ABSTRACT_PUBMED_10390347}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 10390347 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_10390347}}
==About this Structure==
==About this Structure==
-
1B22 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B22 OCA].
+
1B22 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B22 OCA].
==Reference==
==Reference==
Line 32: Line 36:
[[Category: Rsgi]]
[[Category: Rsgi]]
[[Category: Structural genomic]]
[[Category: Structural genomic]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:57:57 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 18:03:36 2008''

Revision as of 15:03, 30 June 2008

Image:1b22.png

Template:STRUCTURE 1b22

RAD51 (N-TERMINAL DOMAIN)

Template:ABSTRACT PUBMED 10390347

About this Structure

1B22 is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

The N-terminal domain of the human Rad51 protein binds DNA: structure and a DNA binding surface as revealed by NMR., Aihara H, Ito Y, Kurumizaka H, Yokoyama S, Shibata T, J Mol Biol. 1999 Jul 9;290(2):495-504. PMID:10390347

Page seeded by OCA on Mon Jun 30 18:03:36 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1b22"
Personal tools