1b3i

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{{STRUCTURE_1b3i| PDB=1b3i | SCENE= }}
{{STRUCTURE_1b3i| PDB=1b3i | SCENE= }}
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'''NMR SOLUTION STRUCTURE OF PLASTOCYANIN FROM THE PHOTOSYNTHETIC PROKARYOTE, PROCHLOROTHRIX HOLLANDICA (MINIMIZED AVERAGE STRUCTURE)'''
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===NMR SOLUTION STRUCTURE OF PLASTOCYANIN FROM THE PHOTOSYNTHETIC PROKARYOTE, PROCHLOROTHRIX HOLLANDICA (MINIMIZED AVERAGE STRUCTURE)===
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==Overview==
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The solution structure of a divergent plastocyanin (PC) from the photosynthetic prokaryote Prochlorothrix hollandica was determined by homonuclear 1H NMR spectroscopy. Nineteen structures were calculated from 1222 distance restraints, yielding a family of structures having an average rmsd of 0.42 +/- 0.08 A for backbone atoms and 0.71 +/- 0.07 A for heavy atoms to the mean structure. No distance constraint was violated by more than 0.26 A in the structure family. Despite the low number of conserved residues shared with other PC homologues, the overall folding pattern of P. hollandica PC is similar to other PCs, in that the protein forms a two-sheet beta-barrel tertiary structure. The greatest variability among the backbone structures is seen in the loop region from residues 47-60. The differences seen in the P. hollandica PC homologue likely arise due to a small deletion of 2-4 residues compared to the PC consensus; this yields a less extended loop containing a short alpha-helix from residues Ala52-Leu55. Additionally, the protein has an altered hydrophobic patch thought to be important in binding reaction partners. Whereas the backbone structure is very similar within the loops of the hydrophobic region, the presence of two unique residues (Tyr12 and Pro14) yields a structurally different hydrophobic surface likely important in binding P. hollandica Photosystem I.
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(as it appears on PubMed at http://www.pubmed.gov), where 10213601 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10213601}}
==About this Structure==
==About this Structure==
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1B3I is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Prochlorothrix_hollandica Prochlorothrix hollandica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B3I OCA].
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1B3I is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Prochlorothrix_hollandica Prochlorothrix hollandica]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B3I OCA].
==Reference==
==Reference==
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[[Category: Photosynthesis]]
[[Category: Photosynthesis]]
[[Category: Type i copper protein]]
[[Category: Type i copper protein]]
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Revision as of 15:09, 30 June 2008

Image:1b3i.png

Template:STRUCTURE 1b3i

NMR SOLUTION STRUCTURE OF PLASTOCYANIN FROM THE PHOTOSYNTHETIC PROKARYOTE, PROCHLOROTHRIX HOLLANDICA (MINIMIZED AVERAGE STRUCTURE)

Template:ABSTRACT PUBMED 10213601

About this Structure

1B3I is a Single protein structure of sequence from Prochlorothrix hollandica. Full experimental information is available from OCA.

Reference

NMR solution structure of plastocyanin from the photosynthetic prokaryote, Prochlorothrix hollandica., Babu CR, Volkman BF, Bullerjahn GS, Biochemistry. 1999 Apr 20;38(16):4988-95. PMID:10213601

Page seeded by OCA on Mon Jun 30 18:09:36 2008

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