1b5f

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{{STRUCTURE_1b5f| PDB=1b5f | SCENE= }}
{{STRUCTURE_1b5f| PDB=1b5f | SCENE= }}
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'''NATIVE CARDOSIN A FROM CYNARA CARDUNCULUS L.'''
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===NATIVE CARDOSIN A FROM CYNARA CARDUNCULUS L.===
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==Overview==
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Aspartic proteinases (AP) have been widely studied within the living world, but so far no plant AP have been structurally characterized. The refined cardosin A crystallographic structure includes two molecules, built up by two glycosylated peptide chains (31 and 15 kDa each). The fold of cardosin A is typical within the AP family. The glycosyl content is described by 19 sugar rings attached to Asn-67 and Asn-257. They are localized on the molecular surface away from the conserved active site and show a new glycan of the plant complex type. A hydrogen bond between Gln-126 and Manbeta4 renders the monosaccharide oxygen O-2 sterically inaccessible to accept a xylosyl residue, therefore explaining the new type of the identified plant glycan. The Arg-Gly-Asp sequence, which has been shown to be involved in recognition of a putative cardosin A receptor, was found in a loop between two beta-strands on the molecular surface opposite the active site cleft. Based on the crystal structure, a possible mechanism whereby cardosin A might be orientated at the cell surface of the style to interact with its putative receptor from pollen is proposed. The biological implications of these findings are also discussed.
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The line below this paragraph, {{ABSTRACT_PUBMED_10488111}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 10488111 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10488111}}
==About this Structure==
==About this Structure==
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[[Category: Carrondo, M A.]]
[[Category: Carrondo, M A.]]
[[Category: Frazao, C.]]
[[Category: Frazao, C.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 11:05:47 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 18:18:32 2008''

Revision as of 15:18, 30 June 2008

Image:1b5f.png

Template:STRUCTURE 1b5f

NATIVE CARDOSIN A FROM CYNARA CARDUNCULUS L.

Template:ABSTRACT PUBMED 10488111

About this Structure

1B5F is a Single protein structure of sequence from Cynara cardunculus. Full crystallographic information is available from OCA.

Reference

Crystal structure of cardosin A, a glycosylated and Arg-Gly-Asp-containing aspartic proteinase from the flowers of Cynara cardunculus L., Frazao C, Bento I, Costa J, Soares CM, Verissimo P, Faro C, Pires E, Cooper J, Carrondo MA, J Biol Chem. 1999 Sep 24;274(39):27694-701. PMID:10488111

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