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1b6i

From Proteopedia

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[[Image:1b6i.gif|left|200px]]
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{{STRUCTURE_1b6i| PDB=1b6i | SCENE= }}
{{STRUCTURE_1b6i| PDB=1b6i | SCENE= }}
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'''T4 LYSOZYME MUTANT WITH CYS 54 REPLACED BY THR, CYS 97 REPLACED BY ALA, THR 21 REPLACED BY CYS AND LYS 124 REPLACED BY CYS (C54T,C97A,T21C,K124C)'''
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===T4 LYSOZYME MUTANT WITH CYS 54 REPLACED BY THR, CYS 97 REPLACED BY ALA, THR 21 REPLACED BY CYS AND LYS 124 REPLACED BY CYS (C54T,C97A,T21C,K124C)===
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==Overview==
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Recent advances in single molecule manipulation methods offer a novel approach to investigating the protein folding problem. These studies usually are done on molecules that are naturally organized as linear arrays of globular domains. To extend these techniques to study proteins that normally exist as monomers, we have developed a method of synthesizing polymers of protein molecules in the solid state. By introducing cysteines at locations where bacteriophage T4 lysozyme molecules contact each other in a crystal and taking advantage of the alignment provided by the lattice, we have obtained polymers of defined polarity up to 25 molecules long that retain enzymatic activity. These polymers then were manipulated mechanically by using a modified scanning force microscope to characterize the force-induced reversible unfolding of the individual lysozyme molecules. This approach should be general and adaptable to many other proteins with known crystal structures. For T4 lysozyme, the force required to unfold the monomers was 64 +/- 16 pN at the pulling speed used. Refolding occurred within 1 sec of relaxation with an efficiency close to 100%. Analysis of the force versus extension curves suggests that the mechanical unfolding transition follows a two-state model. The unfolding forces determined in 1 M guanidine hydrochloride indicate that in these conditions the activation barrier for unfolding is reduced by 2 kcal/mol.
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(as it appears on PubMed at http://www.pubmed.gov), where 10618384 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10618384}}
==About this Structure==
==About this Structure==
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[[Category: Snow, S.]]
[[Category: Snow, S.]]
[[Category: Vetter, I R.]]
[[Category: Vetter, I R.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 18:22:54 2008''

Revision as of 15:22, 30 June 2008

Image:1b6i.png

Template:STRUCTURE 1b6i

T4 LYSOZYME MUTANT WITH CYS 54 REPLACED BY THR, CYS 97 REPLACED BY ALA, THR 21 REPLACED BY CYS AND LYS 124 REPLACED BY CYS (C54T,C97A,T21C,K124C)

Template:ABSTRACT PUBMED 10618384

About this Structure

1B6I is a Single protein structure of sequence from Enterobacteria phage t4. Full crystallographic information is available from OCA.

Reference

Solid-state synthesis and mechanical unfolding of polymers of T4 lysozyme., Yang G, Cecconi C, Baase WA, Vetter IR, Breyer WA, Haack JA, Matthews BW, Dahlquist FW, Bustamante C, Proc Natl Acad Sci U S A. 2000 Jan 4;97(1):139-44. PMID:10618384

Page seeded by OCA on Mon Jun 30 18:22:54 2008

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