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1b79

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{{STRUCTURE_1b79| PDB=1b79 | SCENE= }}
{{STRUCTURE_1b79| PDB=1b79 | SCENE= }}
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'''N-TERMINAL DOMAIN OF DNA REPLICATION PROTEIN DNAB'''
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===N-TERMINAL DOMAIN OF DNA REPLICATION PROTEIN DNAB===
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==Overview==
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BACKGROUND: The hexameric helicase DnaB unwinds the DNA duplex at the Escherichia coli chromosome replication fork. Although the mechanism by which DnaB both couples ATP hydrolysis to translocation along DNA and denatures the duplex is unknown, a change in the quaternary structure of the protein involving dimerization of the N-terminal domain has been observed and may occur during the enzymatic cycle. This N-terminal domain is required both for interaction with other proteins in the primosome and for DnaB helicase activity. Knowledge of the structure of this domain may contribute to an understanding of its role in DnaB function. RESULTS: We have determined the structure of the N-terminal domain of DnaB crystallographically. The structure is globular, highly helical and lacks a close structural relative in the database of known protein folds. Conserved residues and sites of dominant-negative mutations have structurally significant roles. Each asymmetric unit in the crystal contains two independent and identical copies of a dimer of the DnaB N-terminal domain. CONCLUSIONS: The large-scale domain or subunit reorientation that is seen in DnaB by electron microscopy might result from the formation of a true twofold symmetric dimer of N-terminal domains, while maintaining a head-to-tail arrangement of C-terminal domains. The N-terminal domain of DnaB is the first region of a hexameric DNA replicative helicase to be visualized at high resolution. Comparison of this structure to the analogous region of the Rho RNA/DNA helicase indicates that the N-terminal domains of these hexameric helicases are structurally dissimilar.
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(as it appears on PubMed at http://www.pubmed.gov), where 10404598 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10404598}}
==About this Structure==
==About this Structure==
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[[Category: Helicase]]
[[Category: Helicase]]
[[Category: Hexamer]]
[[Category: Hexamer]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 11:09:38 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 18:26:15 2008''

Revision as of 15:26, 30 June 2008

Image:1b79.png

Template:STRUCTURE 1b79

N-TERMINAL DOMAIN OF DNA REPLICATION PROTEIN DNAB

Template:ABSTRACT PUBMED 10404598

About this Structure

1B79 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystal structure of the N-terminal domain of the DnaB hexameric helicase., Fass D, Bogden CE, Berger JM, Structure. 1999 Jun 15;7(6):691-8. PMID:10404598

Page seeded by OCA on Mon Jun 30 18:26:15 2008

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