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| | {{STRUCTURE_1b9i| PDB=1b9i | SCENE= }} | | {{STRUCTURE_1b9i| PDB=1b9i | SCENE= }} |
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| - | '''CRYSTAL STRUCTURE OF 3-AMINO-5-HYDROXYBENZOIC ACID (AHBA) SYNTHASE'''
| + | ===CRYSTAL STRUCTURE OF 3-AMINO-5-HYDROXYBENZOIC ACID (AHBA) SYNTHASE=== |
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| - | ==Overview==
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| - | The biosynthesis of ansamycin antibiotics, including rifamycin B, involves the synthesis of an aromatic precursor, 3-amino-5-hydroxybenzoic acid (AHBA), which serves as starter for the assembly of the antibiotics' polyketide backbone. The terminal enzyme of AHBA formation, AHBA synthase, is a dimeric, pyridoxal 5'-phosphate (PLP) dependent enzyme with pronounced sequence homology to a number of PLP enzymes involved in the biosynthesis of antibiotic sugar moieties. The structure of AHBA synthase from Amycolatopsis mediterranei has been determined to 2.0 A resolution, with bound cofactor, PLP, and in a complex with PLP and an inhibitor (gabaculine). The overall fold of AHBA synthase is similar to that of the aspartate aminotransferase family of PLP-dependent enzymes, with a large domain containing a seven-stranded beta-sheet surrounded by alpha-helices and a smaller domain consisting of a four-stranded antiparallel beta-sheet and four alpha-helices. The uninhibited form of the enzyme shows the cofactor covalently linked to Lys188 in an internal aldimine linkage. On binding the inhibitor, gabaculine, the internal aldimine linkage is broken, and a covalent bond is observed between the cofactor and inhibitor. The active site is composed of residues from two subunits of AHBA synthase, indicating that AHBA synthase is active as a dimer. | + | The line below this paragraph, {{ABSTRACT_PUBMED_10433690}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 10433690 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_10433690}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Scapin, G.]] | | [[Category: Scapin, G.]] |
| | [[Category: Yu, T W.]] | | [[Category: Yu, T W.]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 11:14:37 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 18:37:58 2008'' |
Revision as of 15:38, 30 June 2008
Template:STRUCTURE 1b9i
CRYSTAL STRUCTURE OF 3-AMINO-5-HYDROXYBENZOIC ACID (AHBA) SYNTHASE
Template:ABSTRACT PUBMED 10433690
About this Structure
1B9I is a Single protein structure of sequence from Amycolatopsis mediterranei. Full crystallographic information is available from OCA.
Reference
Crystal structure of 3-amino-5-hydroxybenzoic acid (AHBA) synthase., Eads JC, Beeby M, Scapin G, Yu TW, Floss HG, Biochemistry. 1999 Aug 3;38(31):9840-9. PMID:10433690
Page seeded by OCA on Mon Jun 30 18:37:58 2008
