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1bam

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{{STRUCTURE_1bam| PDB=1bam | SCENE= }}
{{STRUCTURE_1bam| PDB=1bam | SCENE= }}
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'''STRUCTURE OF RESTRICTION ENDONUCLEASE BAMHI PHASED AT 1.95 ANGSTROMS RESOLUTION BY MAD ANALYSIS'''
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===STRUCTURE OF RESTRICTION ENDONUCLEASE BAMHI PHASED AT 1.95 ANGSTROMS RESOLUTION BY MAD ANALYSIS===
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==Overview==
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BACKGROUND: Type II restriction endonucleases recognize DNA sequences that vary between four to eight base pairs, and require only Mg2+ as a cofactor to catalyze the hydrolysis of DNA. Their protein sequences display a surprising lack of similarity, and no recurring structural motif analogous to the helix-turn-helix or the zinc finger of transcription factors, has yet been discovered. RESULTS: We have determined the crystal structure of restriction endonuclease BamHI at 1.95 A resolution. The structure was solved by combining phase information derived from multi-wavelength X-ray data by algebraic and maximum likelihood methods. The BamHI subunit consists of a central beta-sheet with alpha-helices on both sides. The dimer configuration reveals a large cleft which could accommodate B-form DNA. Mutants of the enzyme that are deficient in cleavage are located at or near the putative DNA-binding cleft. BamHI and endonuclease EcoRI share a common core motif (CCM) consisting of five beta-strands and two helices. It remains to be determined if other restriction enzymes also contain the CCM. CONCLUSIONS: The structure of BamHI provides the first clear evidence that there may be substantial structural homology amongst restriction enzymes, even though it is undetectable at the sequence level.
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(as it appears on PubMed at http://www.pubmed.gov), where 8081758 is the PubMed ID number.
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{{ABSTRACT_PUBMED_8081758}}
==About this Structure==
==About this Structure==
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[[Category: Newman, M.]]
[[Category: Newman, M.]]
[[Category: Endonuclease]]
[[Category: Endonuclease]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 11:16:58 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 18:42:49 2008''

Revision as of 15:42, 30 June 2008

Template:STRUCTURE 1bam

STRUCTURE OF RESTRICTION ENDONUCLEASE BAMHI PHASED AT 1.95 ANGSTROMS RESOLUTION BY MAD ANALYSIS

Template:ABSTRACT PUBMED 8081758

About this Structure

1BAM is a Single protein structure of sequence from Bacillus amyloliquefaciens. Full crystallographic information is available from OCA.

Reference

Structure of restriction endonuclease bamhi phased at 1.95 A resolution by MAD analysis., Newman M, Strzelecka T, Dorner LF, Schildkraut I, Aggarwal AK, Structure. 1994 May 15;2(5):439-52. PMID:8081758

Page seeded by OCA on Mon Jun 30 18:42:49 2008

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