1bbi

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[[Image:1bbi.gif|left|200px]]
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{{STRUCTURE_1bbi| PDB=1bbi | SCENE= }}
{{STRUCTURE_1bbi| PDB=1bbi | SCENE= }}
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'''THREE-DIMENSIONAL STRUCTURE OF SOYBEAN TRYPSIN(SLASH)CHYMOTRYPSIN BOWMAN-BIRK INHIBITOR IN SOLUTION'''
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===THREE-DIMENSIONAL STRUCTURE OF SOYBEAN TRYPSIN(SLASH)CHYMOTRYPSIN BOWMAN-BIRK INHIBITOR IN SOLUTION===
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==Overview==
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The three-dimensional structure of soybean trypsin/chymotrypsin Bowman-Birk inhibitor in solution has been determined by two-dimensional 1H nuclear magnetic resonance spectroscopy and dynamical simulated annealing using the program XPLOR. The structure was defined by 907 NOEs involving intra- and interresidue contacts which served as distance constraints for a protocol of dynamical simulated annealing. In addition, 48 phi angle constraints involving non-proline amino acids, 29 chi angle constraints, six omega angle constraints for the X-Pro peptide bond, and 35 stereoassignments for prochiral centers were incorporated during the course of the calculation. The protein is characterized by two distinct binding domains for serine protease. Each domain is comprised of a beta-hairpin (antiparallel beta-sheet and a cis-proline-containing type VIb reverse turn) with a short segment making a third strand of antiparallel beta-sheet. The structure determination and refinement are described, and the structure is compared to other structures of Bowman-Birk inhibitors as well as other families of serine protease inhibitors.
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The line below this paragraph, {{ABSTRACT_PUBMED_1734975}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 1734975 is the PubMed ID number.
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{{ABSTRACT_PUBMED_1734975}}
==About this Structure==
==About this Structure==
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1BBI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Glycine_max Glycine max]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BBI OCA].
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1BBI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Glycine_max Glycine max]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BBI OCA].
==Reference==
==Reference==
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[[Category: Werner, M H.]]
[[Category: Werner, M H.]]
[[Category: Serine protease inhibitor]]
[[Category: Serine protease inhibitor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 11:18:26 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 18:46:07 2008''

Revision as of 15:46, 30 June 2008

Image:1bbi.png

Template:STRUCTURE 1bbi

THREE-DIMENSIONAL STRUCTURE OF SOYBEAN TRYPSIN(SLASH)CHYMOTRYPSIN BOWMAN-BIRK INHIBITOR IN SOLUTION

Template:ABSTRACT PUBMED 1734975

About this Structure

1BBI is a Single protein structure of sequence from Glycine max. Full experimental information is available from OCA.

Reference

Three-dimensional structure of soybean trypsin/chymotrypsin Bowman-Birk inhibitor in solution., Werner MH, Wemmer DE, Biochemistry. 1992 Feb 4;31(4):999-1010. PMID:1734975

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