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1bci

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{{STRUCTURE_1bci| PDB=1bci | SCENE= }}
{{STRUCTURE_1bci| PDB=1bci | SCENE= }}
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'''C2 DOMAIN OF CYTOSOLIC PHOSPHOLIPASE A2, NMR, MINIMIZED AVERAGE STRUCTURE'''
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===C2 DOMAIN OF CYTOSOLIC PHOSPHOLIPASE A2, NMR, MINIMIZED AVERAGE STRUCTURE===
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==Overview==
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The amino-terminal, 138 amino acid C2 domain of cytosolic phospholipase A2 (cPLA2-C2) mediates an initial step in the production of lipid mediators of inflammation: the Ca2+-dependent translocation of the enzyme to intracellular membranes with subsequent liberation of arachidonic acid. The high resolution solution structure of this Ca2+-dependent, lipid-binding domain (CaLB) has been determined using heteronuclear three-dimensional NMR spectroscopy. Secondary structure analysis, derived from several sets of spectroscopic data, shows that the domain is composed of eight antiparallel beta-strands with six interconnecting loops that fits the "type II" topology for C2 domains. Using a total of 2370 distance and torsional restraints, the structure was found to be a beta-sandwich in the "Greek key" motif. The solution structure of cPLA2-C2 domain is very similar to the X-ray crystal structure of the C2 domain of phospholipase-C-delta and phylogenetic analysis clarifies the structural role of highly conserved residues. Calorimetric studies further demonstrate that cPLA2-C2 binds two Ca2+ with observed Kds of approximately 2 microM in an entropically assisted process. Moreover, regions on cPLA2-C2 interacting with membranes were identified by 15N-HSQC-spectroscopy of cPLA2-C2 in the presence of low molecular weight lipid micelles. An extended binding site was identified that binds the phosphocholine headgroup in a Ca2+-dependent manner and also interacts with proximal regions of the membrane surface. Based upon these results, a structural model is presented for the mechanism of association of cPLA2 with its membrane substrate.
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{{ABSTRACT_PUBMED_9665851}}
==About this Structure==
==About this Structure==
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1BCI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BCI OCA].
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1BCI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BCI OCA].
==Reference==
==Reference==
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[[Category: Lipid degradation]]
[[Category: Lipid degradation]]
[[Category: Phosphocholine]]
[[Category: Phosphocholine]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 11:20:25 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 18:49:58 2008''

Revision as of 15:50, 30 June 2008

Image:1bci.png

Template:STRUCTURE 1bci

C2 DOMAIN OF CYTOSOLIC PHOSPHOLIPASE A2, NMR, MINIMIZED AVERAGE STRUCTURE

Template:ABSTRACT PUBMED 9665851

About this Structure

1BCI is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

Solution structure and membrane interactions of the C2 domain of cytosolic phospholipase A2., Xu GY, McDonagh T, Yu HA, Nalefski EA, Clark JD, Cumming DA, J Mol Biol. 1998 Jul 17;280(3):485-500. PMID:9665851

Page seeded by OCA on Mon Jun 30 18:49:58 2008

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