1be4

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{{STRUCTURE_1be4| PDB=1be4 | SCENE= }}
{{STRUCTURE_1be4| PDB=1be4 | SCENE= }}
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'''NUCLEOSIDE DIPHOSPHATE KINASE ISOFORM B FROM BOVINE RETINA'''
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===NUCLEOSIDE DIPHOSPHATE KINASE ISOFORM B FROM BOVINE RETINA===
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==Overview==
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The biochemical and structural properties of bovine retinal nucleoside diphosphate kinase were investigated. The enzyme showed two polypeptides of approximately 17.5 and 18.5 kDa on SDS-PAGE, while isoelectric focusing revealed seven to eight proteins with a pI range of 7.4-8.2. Sedimentation equilibrium yielded a molecular mass of 96 +/- 2 kDa for the enzyme. Carbohydrate analysis revealed that both polypeptides contained Gal, Man, GlcNAc, Fuc, and GalNac saccharides. Like other nucleoside diphosphate kinases, the retinal enzyme showed substantial differences in the Km values for various di- and triphosphate nucleotides. Immunogold labeling of bovine retina revealed that the enzyme is localized on both the membranes and in the cytoplasm. Screening of a retinal cDNA library yielded full-length clones encoding two distinct isoforms (NBR-A and NBR-B). Both isoforms were overexpressed in Escherichia coli and their biochemical properties compared with retinal NDP-kinase. The structures of NBR-A and NBR-B were determined by X-ray crystallography in the presence of guanine nucleotide(s). Both isoforms are hexameric, and the fold of the monomer is similar to other nucleoside diphosphate kinase structures. The NBR-A active site contained both a cGMP and a GDP molecule each bound at half occupancy while the NBR-B active site contained only cGMP.
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{{ABSTRACT_PUBMED_9760230}}
==About this Structure==
==About this Structure==
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[[Category: Tordova, M.]]
[[Category: Tordova, M.]]
[[Category: Phosphotransferase]]
[[Category: Phosphotransferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 11:23:46 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 18:56:59 2008''

Revision as of 15:57, 30 June 2008

Image:1be4.png

Template:STRUCTURE 1be4

NUCLEOSIDE DIPHOSPHATE KINASE ISOFORM B FROM BOVINE RETINA

Template:ABSTRACT PUBMED 9760230

About this Structure

1BE4 is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

Reference

Nucleoside diphosphate kinase from bovine retina: purification, subcellular localization, molecular cloning, and three-dimensional structure., Abdulaev NG, Karaschuk GN, Ladner JE, Kakuev DL, Yakhyaev AV, Tordova M, Gaidarov IO, Popov VI, Fujiwara JH, Chinchilla D, Eisenstein E, Gilliland GL, Ridge KD, Biochemistry. 1998 Oct 6;37(40):13958-67. PMID:9760230

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