1bf6

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{{STRUCTURE_1bf6| PDB=1bf6 | SCENE= }}
{{STRUCTURE_1bf6| PDB=1bf6 | SCENE= }}
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'''PHOSPHOTRIESTERASE HOMOLOGY PROTEIN FROM ESCHERICHIA COLI'''
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===PHOSPHOTRIESTERASE HOMOLOGY PROTEIN FROM ESCHERICHIA COLI===
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==Overview==
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Phosphotriesterase homology protein (PHP) is a member of a recently discovered family of proteins related to phosphotriesterase, a hydrolytic, bacterial enzyme with an unusual substrate specificity for synthetic organophosphate triesters and phosphorofluoridates, which are common constituents of chemical warfare agents and agricultural pesticides. No natural substrate has been identified for phosphotriesterase, and it has been suggested that the enzyme may have evolved the ability to hydrolyze synthetic compounds in bacteria under selective pressure to meet nutritional needs. PHP, which has 28% sequence identity with phosphotriesterase, may belong to the family of proteins from which phosphotriesterase evolved. Here we report the cloning, expression, initial characterization, and high-resolution X-ray crystallographic structure of PHP. Biochemical analysis shows that PHP is monomeric and binds two zinc ions per monomer. Unlike phosphotriesterase, PHP does not catalyze the hydrolysis of nonspecific phosphotriesters. The structure, similar to that of phosphotriesterase, consists of a long, elliptical alpha/beta barrel and has a binuclear zinc center in a cleft at the carboxy end of the barrel at the location of the presumptive active site.
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{{ABSTRACT_PUBMED_9548740}}
==About this Structure==
==About this Structure==
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[[Category: Hypothetical protein]]
[[Category: Hypothetical protein]]
[[Category: Phosphotriesterase]]
[[Category: Phosphotriesterase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 11:26:11 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 19:01:26 2008''

Revision as of 16:01, 30 June 2008

Image:1bf6.png

Template:STRUCTURE 1bf6

PHOSPHOTRIESTERASE HOMOLOGY PROTEIN FROM ESCHERICHIA COLI

Template:ABSTRACT PUBMED 9548740

About this Structure

1BF6 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Biochemical characterization and crystallographic structure of an Escherichia coli protein from the phosphotriesterase gene family., Buchbinder JL, Stephenson RC, Dresser MJ, Pitera JW, Scanlan TS, Fletterick RJ, Biochemistry. 1998 Apr 14;37(15):5096-106. PMID:9548740

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