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| - | [[Image:1bfn.gif|left|200px]] | + | {{Seed}} |
| | + | [[Image:1bfn.png|left|200px]] |
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| | {{STRUCTURE_1bfn| PDB=1bfn | SCENE= }} | | {{STRUCTURE_1bfn| PDB=1bfn | SCENE= }} |
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| - | '''BETA-AMYLASE/BETA-CYCLODEXTRIN COMPLEX'''
| + | ===BETA-AMYLASE/BETA-CYCLODEXTRIN COMPLEX=== |
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| - | ==Overview==
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| - | In order to study the interaction of soybean beta-amylase with substrate, we solved the crystal structure of beta-cyclodextrin-enzyme complex and compared it with that of alpha-cyclodextrin-enzyme complex. The enzyme was expressed in Escherichia coli at a high level as a soluble and catalytically active protein. The purified recombinant enzyme had properties nearly identical to those of native soybean beta-amylase and formed the same crystals as the native enzyme. The crystal structure of recombinant enzyme complexed with beta-cyclodextrin was refined at 2. 07-A resolution with a final crystallographic R value of 15.8% (Rfree = 21.1%). The root mean square deviation in the position of C-alpha atoms between this recombinant enzyme and the native enzyme was 0.22 A. These results indicate that the expression system established here is suitable for studying structure-function relationships of beta-amylase. The conformation of the bound beta-cyclodextrin takes an ellipsoid shape in contrast to the circular shape of the bound alpha-cyclodextrin. The cyclodextrins shared mainly two glucose binding sites, 3 and 4. The glucose residue 4 was slightly shifted from the maltose binding site. This suggests that the binding site of the cyclodextrins is important for its holding of a cleaved substrate, which enables the multiple attack mechanism of beta-amylase.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_9677422}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 9677422 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_9677422}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Hydrolase]] | | [[Category: Hydrolase]] |
| | [[Category: Recombinant]] | | [[Category: Recombinant]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 11:27:01 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 19:03:12 2008'' |
Revision as of 16:03, 30 June 2008
Template:STRUCTURE 1bfn
BETA-AMYLASE/BETA-CYCLODEXTRIN COMPLEX
Template:ABSTRACT PUBMED 9677422
About this Structure
1BFN is a Single protein structure of sequence from Glycine max. Full crystallographic information is available from OCA.
Reference
Crystal structure of recombinant soybean beta-amylase complexed with beta-cyclodextrin., Adachi M, Mikami B, Katsube T, Utsumi S, J Biol Chem. 1998 Jul 31;273(31):19859-65. PMID:9677422
Page seeded by OCA on Mon Jun 30 19:03:12 2008
