1bgs

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{{STRUCTURE_1bgs| PDB=1bgs | SCENE= }}
{{STRUCTURE_1bgs| PDB=1bgs | SCENE= }}
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'''RECOGNITION BETWEEN A BACTERIAL RIBONUCLEASE, BARNASE, AND ITS NATURAL INHIBITOR, BARSTAR'''
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===RECOGNITION BETWEEN A BACTERIAL RIBONUCLEASE, BARNASE, AND ITS NATURAL INHIBITOR, BARSTAR===
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==Overview==
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BACKGROUND: Protein-protein recognition is fundamental to most biological processes. The information we have so far on the interfaces between proteins comes largely from several protease-inhibitor and antigen-antibody complexes. Barnase, a bacterial ribonuclease, and barstar, its natural inhibitor, form a tight complex which provides a good model for the study and design of protein-protein non-covalent interactions. RESULTS: Here we report the structure of a complex between barnase and a fully functional mutant of barstar determined by X-ray analysis. Barstar is composed of three parallel alpha-helices stacked against a three-stranded parallel, beta-sheet, and sterically blocks the active site of the enzyme with an alpha-helix and adjacent loop. The buried surface in the interface between the two molecules totals 1630 A2. The barnase-barstar complex is predominantly stabilized by charge interactions involving positive charges in the active site of the enzyme. Asp39 of barstar binds to the phosphate-binding site of barnase, mimicking enzyme-substrate interactions. CONCLUSION: The phosphate-binding site of the enzyme is the anchor point for inhibitor binding. We propose that this is also likely to be the case for other ribonuclease inhibitors.
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The line below this paragraph, {{ABSTRACT_PUBMED_16100951}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 16100951 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16100951}}
==About this Structure==
==About this Structure==
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[[Category: Mauguen, Y.]]
[[Category: Mauguen, Y.]]
[[Category: Endonuclease]]
[[Category: Endonuclease]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 11:29:31 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 19:07:55 2008''

Revision as of 16:07, 30 June 2008

Image:1bgs.png

Template:STRUCTURE 1bgs

RECOGNITION BETWEEN A BACTERIAL RIBONUCLEASE, BARNASE, AND ITS NATURAL INHIBITOR, BARSTAR

Template:ABSTRACT PUBMED 16100951

About this Structure

1BGS is a Protein complex structure of sequences from Bacillus amyloliquefaciens. Full crystallographic information is available from OCA.

Reference

Recognition between a bacterial ribonuclease, barnase, and its natural inhibitor, barstar., Guillet V, Lapthorn A, Hartley RW, Mauguen Y, Structure. 1993 Nov 15;1(3):165-76. PMID:16100951

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