1bhe

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{{STRUCTURE_1bhe| PDB=1bhe | SCENE= }}
{{STRUCTURE_1bhe| PDB=1bhe | SCENE= }}
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'''POLYGALACTURONASE FROM ERWINIA CAROTOVORA SSP. CAROTOVORA'''
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===POLYGALACTURONASE FROM ERWINIA CAROTOVORA SSP. CAROTOVORA===
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==Overview==
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The crystal structure of the 40-kDa endo-polygalacturonase from Erwinia carotovora ssp. carotovora was solved by multiple isomorphous replacement and refined at 1.9 A to a conventional crystallographic R-factor of 0.198 and Rfree of 0.239. This is the first structure of a polygalacturonase and comprises a 10 turn right-handed parallel beta-helix domain with two loop regions forming a "tunnel like" substrate-binding cleft. Sequence conservation indicates that the active site of polygalacturonase is between these two loop regions, and comparison of the structure of polygalacturonase with that of rhamnogalacturonase A from Aspergillus aculeatus enables two conserved aspartates, presumed to be catalytic residues, to be identified. An adjacent histidine, in accord with biochemical results, is also seen. A similarity in overall electrostatic properties of the substrate-binding clefts of polygalacturonase and pectate lyase, which bind and cleave the same substrate, polygalacturonic acid, is also revealed.
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(as it appears on PubMed at http://www.pubmed.gov), where 9733763 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9733763}}
==About this Structure==
==About this Structure==
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[[Category: Glycosidase]]
[[Category: Glycosidase]]
[[Category: Hydrolyses polygalacturonic acid]]
[[Category: Hydrolyses polygalacturonic acid]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 11:30:59 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 19:10:18 2008''

Revision as of 16:10, 30 June 2008

Image:1bhe.png

Template:STRUCTURE 1bhe

POLYGALACTURONASE FROM ERWINIA CAROTOVORA SSP. CAROTOVORA

Template:ABSTRACT PUBMED 9733763

About this Structure

1BHE is a Single protein structure of sequence from Pectobacterium carotovorum. Full crystallographic information is available from OCA.

Reference

Crystal structure of polygalacturonase from Erwinia carotovora ssp. carotovora., Pickersgill R, Smith D, Worboys K, Jenkins J, J Biol Chem. 1998 Sep 18;273(38):24660-4. PMID:9733763

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