From Proteopedia
(Difference between revisions)
proteopedia linkproteopedia link
|
|
| Line 1: |
Line 1: |
| - | [[Image:1bkb.jpg|left|200px]] | + | {{Seed}} |
| | + | [[Image:1bkb.png|left|200px]] |
| | | | |
| | <!-- | | <!-- |
| Line 9: |
Line 10: |
| | {{STRUCTURE_1bkb| PDB=1bkb | SCENE= }} | | {{STRUCTURE_1bkb| PDB=1bkb | SCENE= }} |
| | | | |
| - | '''INITIATION FACTOR 5A FROM ARCHEBACTERIUM PYROBACULUM AEROPHILUM'''
| + | ===INITIATION FACTOR 5A FROM ARCHEBACTERIUM PYROBACULUM AEROPHILUM=== |
| | | | |
| | | | |
| - | ==Overview==
| + | <!-- |
| - | BACKGROUND: Translation initiation factor 5A (IF-5A) is reported to be involved in the first step of peptide bond formation in translation, to be involved in cell-cycle regulation and to be a cofactor for the Rev and Rex transactivator proteins of human immunodeficiency virus-1 and T-cell leukemia virus I, respectively. IF-5A contains an unusual amino acid, hypusine (N-epsilon-(4-aminobutyl-2-hydroxy)lysine), that is required for its function. The first step in the post-translational modification of lysine to hypusine is catalyzed by the enzyme deoxyhypusine synthase, the structure of which has been published recently. RESULTS: IF-5A from the archebacterium Pyrobaculum aerophilum has been heterologously expressed in Escherichia coli with selenomethionine substitution. The crystal structure of IF-5A has been determined by multiwavelength anomalous diffraction and refined to 1.75 A. Unmodified P. aerophilum IF-5A is found to be a beta structure with two domains and three separate hydrophobic cores. CONCLUSIONS: The lysine (Lys42) that is post-translationally modified by deoxyhypusine synthase is found at one end of the IF-5A molecule in an turn between beta strands beta4 and beta5; this lysine residue is freely solvent accessible. The C-terminal domain is found to be homologous to the cold-shock protein CspA of E. coli, which has a well characterized RNA-binding fold, suggesting that IF-5A is involved in RNA binding.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_9753699}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 9753699 is the PubMed ID number. |
| | + | --> |
| | + | {{ABSTRACT_PUBMED_9753699}} |
| | | | |
| | ==About this Structure== | | ==About this Structure== |
| Line 28: |
Line 32: |
| | [[Category: Waldo, G S.]] | | [[Category: Waldo, G S.]] |
| | [[Category: Translation initiation factor]] | | [[Category: Translation initiation factor]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 11:37:38 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 19:18:05 2008'' |
Revision as of 16:18, 30 June 2008
Template:STRUCTURE 1bkb
INITIATION FACTOR 5A FROM ARCHEBACTERIUM PYROBACULUM AEROPHILUM
Template:ABSTRACT PUBMED 9753699
About this Structure
1BKB is a Single protein structure of sequence from Pyrobaculum aerophilum. Full crystallographic information is available from OCA.
Reference
Structure of translation initiation factor 5A from Pyrobaculum aerophilum at 1.75 A resolution., Peat TS, Newman J, Waldo GS, Berendzen J, Terwilliger TC, Structure. 1998 Sep 15;6(9):1207-14. PMID:9753699
Page seeded by OCA on Mon Jun 30 19:18:05 2008
