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1bp7

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[[Image:1bp7.gif|left|200px]]
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{{STRUCTURE_1bp7| PDB=1bp7 | SCENE= }}
{{STRUCTURE_1bp7| PDB=1bp7 | SCENE= }}
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'''GROUP I MOBILE INTRON ENDONUCLEASE I-CREI COMPLEXED WITH HOMING SITE DNA'''
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===GROUP I MOBILE INTRON ENDONUCLEASE I-CREI COMPLEXED WITH HOMING SITE DNA===
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==Overview==
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The structure of the LAGLIDADG intron-encoded homing endonuclease I-CreI bound to homing site DNA has been determined. The interface is formed by an extended, concave beta sheet from each enzyme monomer that contacts each DNA half-site, resulting in direct side-chain contacts to 18 of the 24 base pairs across the full-length homing site. The structure indicates that I-CreI is optimized to its role in genetic transposition by exhibiting long site-recognition while being able to cleave many closely related target sequences. DNA cleavage is mediated by a compact pair of active sites in the I-CreI homodimer, each of which contains a separate bound divalent cation.
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The line below this paragraph, {{ABSTRACT_PUBMED_9809068}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 9809068 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9809068}}
==About this Structure==
==About this Structure==
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[[Category: Intron homing]]
[[Category: Intron homing]]
[[Category: Laglidadg motif]]
[[Category: Laglidadg motif]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 11:47:34 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 19:29:25 2008''

Revision as of 16:29, 30 June 2008

Image:1bp7.png

Template:STRUCTURE 1bp7

GROUP I MOBILE INTRON ENDONUCLEASE I-CREI COMPLEXED WITH HOMING SITE DNA

Template:ABSTRACT PUBMED 9809068

About this Structure

1BP7 is a Single protein structure of sequence from Chlamydomonas reinhardtii. Full crystallographic information is available from OCA.

Reference

DNA recognition and cleavage by the LAGLIDADG homing endonuclease I-CreI., Jurica MS, Monnat RJ Jr, Stoddard BL, Mol Cell. 1998 Oct;2(4):469-76. PMID:9809068

Page seeded by OCA on Mon Jun 30 19:29:25 2008

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