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| - | [[Image:1bqy.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1bqy| PDB=1bqy | SCENE= }} | | {{STRUCTURE_1bqy| PDB=1bqy | SCENE= }} |
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| - | '''PLASMINOGEN ACTIVATOR (TSV-PA) FROM SNAKE VENOM'''
| + | ===PLASMINOGEN ACTIVATOR (TSV-PA) FROM SNAKE VENOM=== |
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| - | ==Overview==
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| - | BACKGROUND: Trimeresurus stejnejeri venom plasminogen activator (TSV-PA) is a snake venom serine proteinase that specifically activates plasminogen. Snake venom serine proteinases form a subfamily of trypsin-like proteinases that are characterised by a high substrate specificity and resistance to inhibition. Many of these venom enzymes specifically interfere with haemostatic mechanisms and display a long circulating half-life. For these reasons several of them have commercial applications and are potentially attractive pharmacological tools. RESULTS: The crystal structure of TSV-PA has been determined to 2.5 A resolution and refined to an R factor of 17.8 (R free, 24.4). The enzyme, showing the overall polypeptide fold of trypsin-like serine proteinases, displays unique structural elements such as the presence of a phenylalanine at position 193, a C-terminal tail clamped via a disulphide bridge to the 99-loop, and a structurally conserved Asp97 residue. The presence of a cis proline at position 218 is in agreement with evolutionary relationships to glandular kallikrein. CONCLUSIONS: We postulate that Phe 193 accounts for the high substrate specificity of TSV-PA and renders it incapable of forming a stable complex with bovine pancreatic trypsin inhibitor and other extended substrates and inhibitors. Mutational studies previously showed that Asp97 is crucial for the plasminogenolytic activity of TSV-PA, here we identify the conservation of Asp97 in both types of mammalian plasminogen activator - tissue-type (tPA) and urokinase-type (uPA). It seems likely that Asp97 of tPA and uPA will have a similar role in plasminogen recognition. The C-terminal extension of TSV-PA is conserved among snake venom serine proteinases, although its function is unknown. The three-dimensional structure presented here is the first of a snake venom serine proteinase and provides an excellent template for modelling other homologous family members.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_9753698}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 9753698 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_9753698}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Serine proteinase]] | | [[Category: Serine proteinase]] |
| | [[Category: Snake venom]] | | [[Category: Snake venom]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 11:50:58 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 19:33:26 2008'' |
Revision as of 16:33, 30 June 2008
Template:STRUCTURE 1bqy
PLASMINOGEN ACTIVATOR (TSV-PA) FROM SNAKE VENOM
Template:ABSTRACT PUBMED 9753698
About this Structure
1BQY is a Single protein structure of sequence from Viridovipera stejnegeri. Full crystallographic information is available from OCA.
Reference
The crystal structure of the novel snake venom plasminogen activator TSV-PA: a prototype structure for snake venom serine proteinases., Parry MA, Jacob U, Huber R, Wisner A, Bon C, Bode W, Structure. 1998 Sep 15;6(9):1195-206. PMID:9753698
Page seeded by OCA on Mon Jun 30 19:33:26 2008
