1bsr

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{{STRUCTURE_1bsr| PDB=1bsr | SCENE= }}
{{STRUCTURE_1bsr| PDB=1bsr | SCENE= }}
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'''BOVINE SEMINAL RIBONUCLEASE STRUCTURE AT 1.9 ANGSTROMS RESOLUTION'''
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===BOVINE SEMINAL RIBONUCLEASE STRUCTURE AT 1.9 ANGSTROMS RESOLUTION===
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==Overview==
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The crystal structure of bovine seminal ribonuclease, a homodimeric enzyme closely related to pancreatic ribonuclease, has been refined at a nominal resolution of 1.9 A employing data collected on an electronic area detector. The final model consists of two chains containing 1990 non-H atoms, seven sulfate anions and 113 water molecules per asymmetric unit. The unit-cell parameters are a = 36.5 (1), b = 66.7 (1) and c = 107.5 (2) A, space group P22(1)2(1). The R factor is 0.177 for 16 492 reflections in the resolution range 6.0-1.9 A and the deviations from ideal values of bond lengths and bond angles are 0.020 A and 3.7 degrees, respectively. The molecule is formed by two pancreatic like chains, which have their N-terminal segments interchanged so that each active site is formed by residues from both subunits. The two chains are related by a non-crystallographic twofold symmetry and are covalently linked by two consecutive disulfide bridges, which form an unusual sixteen-membered ring across the dimer interface. The deviations from the molecular symmetry, the hydration shell and the sulfate-binding sites are also discussed in relation to the known structure of the pancreatic enzyme.
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(as it appears on PubMed at http://www.pubmed.gov), where 15299514 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15299514}}
==About this Structure==
==About this Structure==
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[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Mazzarella, L.]]
[[Category: Mazzarella, L.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 11:54:54 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 19:40:01 2008''

Revision as of 16:40, 30 June 2008

Image:1bsr.png

Template:STRUCTURE 1bsr

BOVINE SEMINAL RIBONUCLEASE STRUCTURE AT 1.9 ANGSTROMS RESOLUTION

Template:ABSTRACT PUBMED 15299514

About this Structure

1BSR is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

Reference

Bovine seminal ribonuclease: structure at 1.9 A resolution., Mazzarella L, Capasso S, Demasi D, Di Lorenzo G, Mattia CA, Zagari A, Acta Crystallogr D Biol Crystallogr. 1993 Jul 1;49(Pt 4):389-402. PMID:15299514

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