1bss

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[[Image:1bss.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_1bss| PDB=1bss | SCENE= }}
{{STRUCTURE_1bss| PDB=1bss | SCENE= }}
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'''ECORV-T93A/DNA/CA2+'''
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===ECORV-T93A/DNA/CA2+===
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==Overview==
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The 2.15-A resolution cocrystal structure of EcoRV endonuclease mutant T93A complexed with DNA and Ca2+ ions reveals two divalent metals bound in one of the active sites. One of these metals is ligated through an inner-sphere water molecule to the phosphate group located 3' to the scissile phosphate. A second inner-sphere water on this metal is positioned approximately in-line for attack on the scissile phosphate. This structure corroborates the observation that the pro-SP phosphoryl oxygen on the adjacent 3' phosphate cannot be modified without severe loss of catalytic efficiency. The structural equivalence of key groups, conserved in the active sites of EcoRV, EcoRI, PvuII, and BamHI endonucleases, suggests that ligation of a catalytic divalent metal ion to this phosphate may occur in many type II restriction enzymes. Together with previous cocrystal structures, these data allow construction of a detailed model for the pretransition state configuration in EcoRV. This model features three divalent metal ions per active site and invokes assistance in the bond-making step by a conserved lysine, which stabilizes the attacking hydroxide ion nucleophile.
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(as it appears on PubMed at http://www.pubmed.gov), where 9811827 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9811827}}
==About this Structure==
==About this Structure==
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[[Category: Horton, N C.]]
[[Category: Horton, N C.]]
[[Category: Perona, J J.]]
[[Category: Perona, J J.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 11:54:58 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 19:40:05 2008''

Revision as of 16:40, 30 June 2008

Image:1bss.png

Template:STRUCTURE 1bss

ECORV-T93A/DNA/CA2+

Template:ABSTRACT PUBMED 9811827

About this Structure

1BSS is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Metal ion-mediated substrate-assisted catalysis in type II restriction endonucleases., Horton NC, Newberry KJ, Perona JJ, Proc Natl Acad Sci U S A. 1998 Nov 10;95(23):13489-94. PMID:9811827

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