1but

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{{STRUCTURE_1but| PDB=1but | SCENE= }}
{{STRUCTURE_1but| PDB=1but | SCENE= }}
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'''NMR STRUCTURE OF THE DNA DECAMER D(CATGGCCATG)2, 10 STRUCTURES'''
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===NMR STRUCTURE OF THE DNA DECAMER D(CATGGCCATG)2, 10 STRUCTURES===
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==Overview==
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The solution structure of the DNA decamer (CATGGCCATG)2 has been determined by NMR spectroscopy and restrained molecular dynamic and distance geometry calculations. The restrainted data set includes interproton distances and torsion angles for the deoxyribose sugar ring which were obtained by nuclear Overhauser enhancement intensities and quantitative simulation of cross-peaks from double quantum filtered correlation spectroscopy. The backbone torsion angles were constrained using experimental data from NOE cross-peaks, 1H-1H and 1H-31P-coupling constants. The NMR structure and the crystal structure of the DNA decamer deviates from the structure of the canonical form of B-DNA in a number of observable characteristics. Particularly, both structures display a specific pattern of stacking interaction in the central GGC base triplet. Furthermore, a specific local conformation of the TG/CA base-pair step is present in NMR and crystal structure, highlighting the unusually high flexibility of this DNA duplex part. The solution structure of the TG/CA base-pair step obtained by our high resolution NMR study is characterized by a positive roll angle, whereas in crystal this base-pair step tends to adopt remarkably high twist angles.
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(as it appears on PubMed at http://www.pubmed.gov), where 9878363 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9878363}}
==About this Structure==
==About this Structure==
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BUT OCA].
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Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BUT OCA].
==Reference==
==Reference==
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[[Category: Deoxyribonucleic acid]]
[[Category: Deoxyribonucleic acid]]
[[Category: Dna]]
[[Category: Dna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 11:58:46 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 19:45:07 2008''

Revision as of 16:45, 30 June 2008

Image:1but.png

Template:STRUCTURE 1but

NMR STRUCTURE OF THE DNA DECAMER D(CATGGCCATG)2, 10 STRUCTURES

Template:ABSTRACT PUBMED 9878363

About this Structure

Full experimental information is available from OCA.

Reference

Structure determination and analysis of helix parameters in the DNA decamer d(CATGGCCATG)2 comparison of results from NMR and crystallography., Dornberger U, Flemming J, Fritzsche H, J Mol Biol. 1998 Dec 18;284(5):1453-63. PMID:9878363

Page seeded by OCA on Mon Jun 30 19:45:07 2008

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