1eiz
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(New page: 200px<br /><applet load="1eiz" size="450" color="white" frame="true" align="right" spinBox="true" caption="1eiz, resolution 1.7Å" /> '''FTSJ RNA METHYLTRANSF...)
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Revision as of 11:55, 20 November 2007
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FTSJ RNA METHYLTRANSFERASE COMPLEXED WITH S-ADENOSYLMETHIONINE
Overview
Structural, biochemical, and genetic techniques were applied to, investigate the function of FtsJ, a recently identified heat shock, protein. FtsJ is well conserved, from bacteria to humans. The 1.5 A, crystal structure of FtsJ in complex with its cofactor, S-adenosylmethionine revealed that FtsJ has a methyltransferase fold. The, molecular surface of FtsJ exposes a putative nucleic acid binding groove, composed of highly conserved, positively charged residues. Substrate, analysis showed that FtsJ methylates 23S rRNA within 50S ribosomal, subunits in vitro and in vivo. Null mutations in ftsJ show a dramatically, altered ribosome profile, a severe growth disadvantage, and a, temperature-sensitive phenotype. Our results reveal an unexpected link, between the heat shock response and RNA metabolism.
About this Structure
1EIZ is a Single protein structure of sequence from Escherichia coli with SAM as ligand. Full crystallographic information is available from OCA.
Reference
RNA methylation under heat shock control., Bugl H, Fauman EB, Staker BL, Zheng F, Kushner SR, Saper MA, Bardwell JC, Jakob U, Mol Cell. 2000 Aug;6(2):349-60. PMID:10983982
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