1ej1
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /><applet load="1ej1" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ej1, resolution 2.2Å" /> '''COCRYSTAL STRUCTURE O...)
Next diff →
Revision as of 11:55, 20 November 2007
|
COCRYSTAL STRUCTURE OF THE MESSENGER RNA 5' CAP-BINDING PROTEIN (EIF4E) BOUND TO 7-METHYL-GDP
Overview
The X-ray structure of the eukaryotic translation initiation factor 4E, (eIF4E), bound to 7-methyl-GDP, has been determined at 2.2 A resolution., eIF4E recognizes 5' 7-methyl-G(5')ppp(5')N mRNA caps during the, rate-limiting initiation step of translation. The protein resembles a, cupped hand and consists of a curved, 8-stranded antiparallel beta sheet, backed by three long alpha helices. 7-methyl-GDP binds in a narrow, cap-binding slot on the molecule's concave surface, where 7-methyl-guanine, recognition is mediated by base sandwiching between two conserved, tryptophans, plus formation of three hydrogen bonds and a van der Waals, contact between its N7-methyl group and a third conserved tryptophan. The, convex dorsal surface of the molecule displays a phylogenetically, conserved hydrophobic/acidic portion, which may interact with other, translation initiation factors and regulatory proteins.
About this Structure
1EJ1 is a Single protein structure of sequence from Mus musculus with M7G as ligand. Full crystallographic information is available from OCA.
Reference
Cocrystal structure of the messenger RNA 5' cap-binding protein (eIF4E) bound to 7-methyl-GDP., Marcotrigiano J, Gingras AC, Sonenberg N, Burley SK, Cell. 1997 Jun 13;89(6):951-61. PMID:9200613
Page seeded by OCA on Tue Nov 20 14:02:27 2007
