1bvu

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[[Image:1bvu.gif|left|200px]]
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{{STRUCTURE_1bvu| PDB=1bvu | SCENE= }}
{{STRUCTURE_1bvu| PDB=1bvu | SCENE= }}
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'''GLUTAMATE DEHYDROGENASE FROM THERMOCOCCUS LITORALIS'''
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===GLUTAMATE DEHYDROGENASE FROM THERMOCOCCUS LITORALIS===
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==Overview==
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Glutamate dehydrogenase catalyses the oxidative deamination of glutamate to 2-oxoglutarate with concomitant reduction of NAD(P)(+), and has been shown to be widely distributed in nature across species ranging from psychrophiles to hyperthermophiles. Extensive characterisation of this enzyme isolated from hyperthermophilic organisms has led to its adoption as a model system for analysing the determinants of thermal stability. The crystal structure of the extremely thermostable glutamate dehydrogenase from Thermococcus litoralis has been determined at 2.5 A resolution, and has been compared to that from the hyperthermophile Pyrococcus furiosus. The two enzymes are 87 % identical in sequence, yet differ 16-fold in their half-lives at 104 degrees C. This is the first reported comparative analysis of the structures of a multisubunit enzyme from two closely related yet distinct hyperthermophilies. The less stable T. litoralis enzyme has a decreased number of ion pair interactions; modified patterns of hydrogen bonding resulting from isosteric sequence changes; substitutions that decrease packing efficiency; and substitutions which give rise to subtle but distinct shifts in both main-chain and side-chain elements of the structure. This analysis provides a rational basis to test ideas on the factors that confer thermal stability in proteins through a combination of mutagenesis, calorimetry, and structural studies.
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(as it appears on PubMed at http://www.pubmed.gov), where 10547290 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10547290}}
==About this Structure==
==About this Structure==
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[[Category: Oxidoreductase]]
[[Category: Oxidoreductase]]
[[Category: Thermal stability]]
[[Category: Thermal stability]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 12:00:59 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 19:47:59 2008''

Revision as of 16:48, 30 June 2008

Image:1bvu.png

Template:STRUCTURE 1bvu

GLUTAMATE DEHYDROGENASE FROM THERMOCOCCUS LITORALIS

Template:ABSTRACT PUBMED 10547290

About this Structure

1BVU is a Single protein structure of sequence from Thermococcus litoralis. Full crystallographic information is available from OCA.

Reference

Structure determination of the glutamate dehydrogenase from the hyperthermophile Thermococcus litoralis and its comparison with that from Pyrococcus furiosus., Britton KL, Yip KS, Sedelnikova SE, Stillman TJ, Adams MW, Ma K, Maeder DL, Robb FT, Tolliday N, Vetriani C, Rice DW, Baker PJ, J Mol Biol. 1999 Nov 12;293(5):1121-32. PMID:10547290

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