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1bvw

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{{STRUCTURE_1bvw| PDB=1bvw | SCENE= }}
{{STRUCTURE_1bvw| PDB=1bvw | SCENE= }}
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'''CELLOBIOHYDROLASE II (CEL6A) FROM HUMICOLA INSOLENS'''
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===CELLOBIOHYDROLASE II (CEL6A) FROM HUMICOLA INSOLENS===
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==Overview==
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The three-dimensional structure of the catalytic core of the family 6 cellobiohydrolase II, Cel6A (CBH II), from Humicola insolens has been determined by X-ray crystallography at a resolution of 1.92 A. The structure was solved by molecular replacement using the homologous Trichoderma reesei CBH II as a search model. The H. insolens enzyme displays a high degree of structural similarity with its T. reesei equivalent. The structure features both O- (alpha-linked mannose) and N-linked glycosylation and a hexa-co-ordinate Mg2+ ion. The active-site residues are located within the enclosed tunnel that is typical for cellobiohydrolase enzymes and which may permit a processive hydrolysis of the cellulose substrate. The close structural similarity between the two enzymes implies that kinetics and chain-end specificity experiments performed on the H. insolens enzyme are likely to be applicable to the homologous T. reesei enzyme. These cast doubt on the description of cellobiohydrolases as exo-enzymes since they demonstrated that Cel6A (CBH II) shows no requirement for non-reducing chain-ends, as had been presumed. There is no crystallographic evidence in the present structure to support a mechanism involving loop opening, yet preliminary modelling experiments suggest that the active-site tunnel of Cel6A (CBH II) is too narrow to permit entry of a fluorescenyl-derivatized substrate, known to be a viable substrate for this enzyme.
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==About this Structure==
==About this Structure==
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[[Category: Cellulose degradation]]
[[Category: Cellulose degradation]]
[[Category: Glycoside hydrolase family 6]]
[[Category: Glycoside hydrolase family 6]]
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Revision as of 16:48, 30 June 2008

Image:1bvw.png

Template:STRUCTURE 1bvw

CELLOBIOHYDROLASE II (CEL6A) FROM HUMICOLA INSOLENS

Template:ABSTRACT PUBMED 9882628

About this Structure

1BVW is a Single protein structure of sequence from Humicola insolens. Full crystallographic information is available from OCA.

Reference

Crystal structure of the catalytic core domain of the family 6 cellobiohydrolase II, Cel6A, from Humicola insolens, at 1.92 A resolution., Varrot A, Hastrup S, Schulein M, Davies GJ, Biochem J. 1999 Jan 15;337 ( Pt 2):297-304. PMID:9882628

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