1c04

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{{STRUCTURE_1c04| PDB=1c04 | SCENE= }}
{{STRUCTURE_1c04| PDB=1c04 | SCENE= }}
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'''IDENTIFICATION OF KNOWN PROTEIN AND RNA STRUCTURES IN A 5 A MAP OF THE LARGE RIBOSOMAL SUBUNIT FROM HALOARCULA MARISMORTUI'''
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===IDENTIFICATION OF KNOWN PROTEIN AND RNA STRUCTURES IN A 5 A MAP OF THE LARGE RIBOSOMAL SUBUNIT FROM HALOARCULA MARISMORTUI===
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==Overview==
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We have calculated at 5.0 A resolution an electron-density map of the large 50S ribosomal subunit from the bacterium Haloarcula marismortui by using phases derived from four heavy-atom derivatives, intercrystal density averaging and density-modification procedures. More than 300 base pairs of A-form RNA duplex have been fitted into this map, as have regions of non-A-form duplex, single-stranded segments and tetraloops. The long rods of RNA crisscrossing the subunit arise from the stacking of short, separate double helices, not all of which are A-form, and in many places proteins crosslink two or more of these rods. The polypeptide exit channel was marked by tungsten cluster compounds bound in one heavy-atom-derivatized crystal. We have determined the structure of the translation-factor-binding centre by fitting the crystal structures of the ribosomal proteins L6, L11 and L14, the sarcin-ricin loop RNA, and the RNA sequence that binds L11 into the electron density. We can position either elongation factor G or elongation factor Tu complexed with an aminoacylated transfer RNA and GTP onto the factor-binding centre in a manner that is consistent with results from biochemical and electron microscopy studies.
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(as it appears on PubMed at http://www.pubmed.gov), where 10476961 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10476961}}
==About this Structure==
==About this Structure==
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[[Category: Large ribosome unit]]
[[Category: Large ribosome unit]]
[[Category: Low resolution model]]
[[Category: Low resolution model]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 12:10:29 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 20:01:28 2008''

Revision as of 17:01, 30 June 2008

Template:STRUCTURE 1c04

IDENTIFICATION OF KNOWN PROTEIN AND RNA STRUCTURES IN A 5 A MAP OF THE LARGE RIBOSOMAL SUBUNIT FROM HALOARCULA MARISMORTUI

Template:ABSTRACT PUBMED 10476961

About this Structure

1C04 is a Protein complex structure of sequences from Haloarcula marismortui. Full crystallographic information is available from OCA.

Reference

Placement of protein and RNA structures into a 5 A-resolution map of the 50S ribosomal subunit., Ban N, Nissen P, Hansen J, Capel M, Moore PB, Steitz TA, Nature. 1999 Aug 26;400(6747):841-7. PMID:10476961

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