1c0b

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{{Seed}}
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{{STRUCTURE_1c0b| PDB=1c0b | SCENE= }}
{{STRUCTURE_1c0b| PDB=1c0b | SCENE= }}
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'''BOVINE PANCREATIC RIBONUCLEASE A DESICCATED FOR 2.5 DAYS'''
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===BOVINE PANCREATIC RIBONUCLEASE A DESICCATED FOR 2.5 DAYS===
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==Overview==
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Unlike most protein crystals, form IX of bovine pancreatic ribonuclease A diffracts well when severely dehydrated. Crystal structures have been solved after 2.5 and 4 days of desiccation with CaSO4, at 1.9 and 2.0 A resolution, respectively. The two desiccated structures are very similar. An RMS displacement of 1.6 A is observed for main-chain atoms in each structure when compared to the hydrated crystal structure with some large rearrangements observed in loop regions. The structural changes are the result of intermolecular contacts formed by strong electrostatic interactions in the absence of a high dielectric medium. The electron density is very diffuse for some surface loops, consistent with a very disordered structure. This disorder is related to the conformational changes. These results help explain conformational changes during the lyophilization of protein and the associated phenomena of denaturation and molecular memory.
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(as it appears on PubMed at http://www.pubmed.gov), where 10548049 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10548049}}
==About this Structure==
==About this Structure==
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[[Category: Desiccated]]
[[Category: Desiccated]]
[[Category: Dry]]
[[Category: Dry]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 12:11:00 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 20:02:04 2008''

Revision as of 17:02, 30 June 2008

Image:1c0b.png

Template:STRUCTURE 1c0b

BOVINE PANCREATIC RIBONUCLEASE A DESICCATED FOR 2.5 DAYS

Template:ABSTRACT PUBMED 10548049

About this Structure

1C0B is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

Reference

X-ray crystal structures of a severely desiccated protein., Bell JA, Protein Sci. 1999 Oct;8(10):2033-40. PMID:10548049

Page seeded by OCA on Mon Jun 30 20:02:04 2008

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