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1c15

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[[Image:1c15.gif|left|200px]]
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{{STRUCTURE_1c15| PDB=1c15 | SCENE= }}
{{STRUCTURE_1c15| PDB=1c15 | SCENE= }}
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'''SOLUTION STRUCTURE OF APAF-1 CARD'''
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===SOLUTION STRUCTURE OF APAF-1 CARD===
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==Overview==
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Direct recruitment and activation of caspase-9 by Apaf-1 through the homophilic CARD/CARD (Caspase Recruitment Domain) interaction is critical for the activation of caspases downstream of mitochondrial damage in apoptosis. Here we report the solution structure of the Apaf-1 CARD domain and its surface of interaction with caspase-9 CARD. Apaf-1 CARD consists of six tightly packed amphipathic alpha-helices and is topologically similar to the RAIDD CARD, with the exception of a kink observed in the middle of the N-terminal helix. By using chemical shift perturbation data, the homophilic interaction was mapped to the acidic surface of Apaf-1 CARD centered around helices 2 and 3. Interestingly, a significant portion of the chemically perturbed residues are hydrophobic, indicating that in addition to the electrostatic interactions predicted previously, hydrophobic interaction is also an important driving force underlying the CARD/CARD interaction. On the basis of the identified functional residues of Apaf-1 CARD and the surface charge complementarity, we propose a model of CARD/CARD interaction between Apaf-1 and caspase-9.
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(as it appears on PubMed at http://www.pubmed.gov), where 10500165 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10500165}}
==About this Structure==
==About this Structure==
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1C15 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C15 OCA].
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1C15 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C15 OCA].
==Reference==
==Reference==
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[[Category: Homophilic interaction]]
[[Category: Homophilic interaction]]
[[Category: Programmed cell death]]
[[Category: Programmed cell death]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 12:12:28 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 20:03:57 2008''

Revision as of 17:03, 30 June 2008

Image:1c15.png

Template:STRUCTURE 1c15

SOLUTION STRUCTURE OF APAF-1 CARD

Template:ABSTRACT PUBMED 10500165

About this Structure

1C15 is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

Solution structure of Apaf-1 CARD and its interaction with caspase-9 CARD: a structural basis for specific adaptor/caspase interaction., Zhou P, Chou J, Olea RS, Yuan J, Wagner G, Proc Natl Acad Sci U S A. 1999 Sep 28;96(20):11265-70. PMID:10500165

Page seeded by OCA on Mon Jun 30 20:03:57 2008

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