1c4l

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[[Image:1c4l.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_1c4l| PDB=1c4l | SCENE= }}
{{STRUCTURE_1c4l| PDB=1c4l | SCENE= }}
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'''SOLUTION STRUCTURE OF AN RNA DUPLEX INCLUDING A C-U BASE-PAIR'''
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===SOLUTION STRUCTURE OF AN RNA DUPLEX INCLUDING A C-U BASE-PAIR===
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==Overview==
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The formation of the C-U base pair in a duplex was observed in solution by means of the temperature profile of (15)N chemical shifts, and the precise geometry of the C-U base pair was also determined by NOE-based structure calculation. From the solution structure of the RNA oligomer, r[CGACUCAGG].r[CCUGCGUCG], it was found that a single C-U mismatch preferred being stacked in the duplex rather than being flipped-out even in solution. Moreover, it adopts an irregular geometry, where the amino nitrogen (N4) of the cytidine and keto-oxygen (O4) of the uridine are within hydrogen-bonding distance, as seen in crystals. To further prove the presence of a hydrogen bond in the C-U pair, we employed a point-labeled cytidine at the exocyclic amino nitrogen of the cytidine in the C-U pair. The temperature profile of its (15)N chemical shift showed a sigmoidal transition curve, indicating the presence of a hydrogen bond in the C-U pair in the duplex.
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The line below this paragraph, {{ABSTRACT_PUBMED_10852704}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 10852704 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10852704}}
==About this Structure==
==About this Structure==
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C4L OCA].
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Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C4L OCA].
==Reference==
==Reference==
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[[Category: Yasuno, K.]]
[[Category: Yasuno, K.]]
[[Category: C-u base pair]]
[[Category: C-u base pair]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 12:19:17 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 20:12:03 2008''

Revision as of 17:12, 30 June 2008

Image:1c4l.png

Template:STRUCTURE 1c4l

SOLUTION STRUCTURE OF AN RNA DUPLEX INCLUDING A C-U BASE-PAIR

Template:ABSTRACT PUBMED 10852704

About this Structure

Full experimental information is available from OCA.

Reference

Solution structure of an RNA duplex including a C-U base pair., Tanaka Y, Kojima C, Yamazaki T, Kodama TS, Yasuno K, Miyashita S, Ono A, Ono A, Kainosho M, Kyogoku Y, Biochemistry. 2000 Jun 20;39(24):7074-80. PMID:10852704

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