1c56

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{{STRUCTURE_1c56| PDB=1c56 | SCENE= }}
{{STRUCTURE_1c56| PDB=1c56 | SCENE= }}
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'''NMR SOLUTION STRUCTURE OF BUTANTOXIN'''
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===NMR SOLUTION STRUCTURE OF BUTANTOXIN===
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==Overview==
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The NMR structure of a new toxin, butantoxin (BuTX), which is present in the venoms of the three Brazilian scorpions Tityus serrulatus, Tityus bahiensis, and Tityus stigmurus, has been investigated. This toxin was shown to reversibly block the Shaker B potassium channels (K(d) approximately 660 nM) and inhibit the proliferation of T-cells and the interleukin-2 production of antigen-stimulated T-helper cells. BuTX is a 40 amino acid basic protein stabilized by the four disulfide bridges: Cys2-Cys5, Cys10-Cys31, Cys16-Cys36, and Cys20-Cys38. The latter three are conserved among all members of the short-chain scorpion toxin family, while the first is unique to BuTX. The three-dimensional structure of BuTX was determined using (1)H-NMR spectroscopy. NOESY, phase sensitive COSY (PH-COSY), and amide hydrogen exchange data were used to generate constraints for molecular modeling calculations. Distance geometry and simulated annealing calculations were performed to generate a family of 49 structures free of constraint violations. The secondary structure of BuTX consists of a short 2(1/2) turn alpha-helix (Glu15-Phe23) and a beta-sheet. The beta-sheet is composed of two well-defined antiparallel strands (Gly29-Met32 and Lys35-Cys38) connected by a type-I' beta-turn (Asn33-Asn34). Residues Cys5-Ala9 form a quasi-third strand of the beta-sheet. The N-terminal C2-C5 disulfide bridge unique to this toxin does not appear to confer stability to the protein.
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(as it appears on PubMed at http://www.pubmed.gov), where 10864437 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10864437}}
==About this Structure==
==About this Structure==
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1C56 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Tityus_serrulatus Tityus serrulatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C56 OCA].
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1C56 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Tityus_serrulatus Tityus serrulatus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C56 OCA].
==Reference==
==Reference==
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[[Category: Nmr structure]]
[[Category: Nmr structure]]
[[Category: Toxin]]
[[Category: Toxin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 12:20:29 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 20:13:29 2008''

Revision as of 17:13, 30 June 2008

Image:1c56.png

Template:STRUCTURE 1c56

NMR SOLUTION STRUCTURE OF BUTANTOXIN

Template:ABSTRACT PUBMED 10864437

About this Structure

1C56 is a Single protein structure of sequence from Tityus serrulatus. Full experimental information is available from OCA.

Reference

NMR solution structure of butantoxin., Holaday SK Jr, Martin BM, Fletcher PL Jr, Krishna NR, Arch Biochem Biophys. 2000 Jul 1;379(1):18-27. PMID:10864437

Page seeded by OCA on Mon Jun 30 20:13:29 2008

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