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1c7k

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{{STRUCTURE_1c7k| PDB=1c7k | SCENE= }}
{{STRUCTURE_1c7k| PDB=1c7k | SCENE= }}
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'''CRYSTAL STRUCTURE OF THE ZINC PROTEASE'''
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===CRYSTAL STRUCTURE OF THE ZINC PROTEASE===
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==Overview==
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A zinc endoprotease produced by Streptomyces caespitosus (ScNP) contains a H83E84TGH87VLG90LPD93-Met103 sequence. Except for D93, this amino acid sequence is the same as a characteristic consensus HEXXHXXGXXH-M motif found in one class of zinc endoprotease called 'metzincins'. We analyzed the structural and functional role of the consensus sequence located around a catalytically essential zinc ion based on the crystal structure of ScNP. The structure was determined at the highest level on resolution (1 A resolution) and accuracy among crystal structures of zinc endoproteases ever determined. The zinc ion of ScNP is tetrahedrally coordinated by three amino acid side-chains (H83, H87 and D93) and a water molecule. The distances between the zinc ion and the coordinating atoms are 2.01, 2.01 and 1.95 A for H83N epsilon, H87N epsilon and D93O delta, respectively. These distances agree very well with those normally found in crystal structures of zinc-containing small molecules in the Cambridge Structural Database. On the other hand, the distance between the zinc ion and the coordinating water molecule (1.93 A) is slightly shorter than the typical value (2.01 A) found in the database. In addition, E84O epsilon makes a short hydrogen bond to this water molecule with the distance of 2.54 A. Two hydrogen bonds (H83N delta-L102O, H87N delta-L91O) and van der Waals interactions between the side-chain of M103 and the two imidazole rings of H83 and H87 are also observed. These interactions are probably important for the imidazole rings to construct the tetrahedral coordination arrangement toward the zinc ion.
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(as it appears on PubMed at http://www.pubmed.gov), where 11132632 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11132632}}
==About this Structure==
==About this Structure==
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[[Category: Alpha and beta protein]]
[[Category: Alpha and beta protein]]
[[Category: Metalloproteinase]]
[[Category: Metalloproteinase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 12:25:25 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 20:19:27 2008''

Revision as of 17:19, 30 June 2008

Image:1c7k.png

Template:STRUCTURE 1c7k

CRYSTAL STRUCTURE OF THE ZINC PROTEASE

Template:ABSTRACT PUBMED 11132632

About this Structure

1C7K is a Single protein structure of sequence from Streptomyces caespitosus. Full crystallographic information is available from OCA.

Reference

Structure of the zinc-binding site in the crystal structure of a zinc endoprotease from Streptomyces caespitosus at 1 A resolution., Kurisu G, Kai Y, Harada S, J Inorg Biochem. 2000 Nov;82(1-4):225-8. PMID:11132632

Page seeded by OCA on Mon Jun 30 20:19:27 2008

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