1c8b

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{{STRUCTURE_1c8b| PDB=1c8b | SCENE= }}
{{STRUCTURE_1c8b| PDB=1c8b | SCENE= }}
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'''CRYSTAL STRUCTURE OF A NOVEL GERMINATION PROTEASE FROM SPORES OF BACILLUS MEGATERIUM: STRUCTURAL REARRANGEMENTS AND ZYMOGEN ACTIVATION'''
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===CRYSTAL STRUCTURE OF A NOVEL GERMINATION PROTEASE FROM SPORES OF BACILLUS MEGATERIUM: STRUCTURAL REARRANGEMENTS AND ZYMOGEN ACTIVATION===
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==Overview==
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The DNA in the core of spores of Bacillus species is saturated with a group of small, acid-soluble proteins (SASP) that protect DNA from a variety of harsh treatments and play a major role in spore resistance and long-term spore survival. During spore germination, SASPs are rapidly degraded to amino acids and this degradation is initiated by a sequence-specific protease called germination protease (GPR), which exhibits no obvious mechanistic or amino acid sequence similarity to any known class of proteases. GPR is synthesized during sporulation as an inactive tetrameric zymogen termed P(46), which later autoprocesses to a smaller form termed P(41), which is active only during spore germination. Here, we report the crystal structure of P(46) from Bacillus megaterium at 3.0 A resolution and the fact that P(46) monomer adopts a novel fold. The asymmetric unit contains two P(46) monomers and the functional tetramer is a dimer of dimers, with an approximately 9 A channel in the center of the tetramer. Analysis of the P(46) structure and site-directed mutagenesis studies have provided some insight into the mechanism of zymogen activation as well as the zymogen's lack of activity and the inactivity of P(41) in the mature spore.
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{{ABSTRACT_PUBMED_10864493}}
==About this Structure==
==About this Structure==
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[[Category: Setlow, P.]]
[[Category: Setlow, P.]]
[[Category: Novel fold]]
[[Category: Novel fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 20:21:22 2008''

Revision as of 17:21, 30 June 2008

Image:1c8b.png

Template:STRUCTURE 1c8b

CRYSTAL STRUCTURE OF A NOVEL GERMINATION PROTEASE FROM SPORES OF BACILLUS MEGATERIUM: STRUCTURAL REARRANGEMENTS AND ZYMOGEN ACTIVATION

Template:ABSTRACT PUBMED 10864493

About this Structure

1C8B is a Single protein structure of sequence from Bacillus megaterium. Full crystallographic information is available from OCA.

Reference

Crystal structure of a novel germination protease from spores of Bacillus megaterium: structural arrangement and zymogen activation., Ponnuraj K, Rowland S, Nessi C, Setlow P, Jedrzejas MJ, J Mol Biol. 2000 Jun 30;300(1):1-10. PMID:10864493

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