1c8i

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{{STRUCTURE_1c8i| PDB=1c8i | SCENE= }}
{{STRUCTURE_1c8i| PDB=1c8i | SCENE= }}
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'''BINDING MODE OF HYDROXYLAMINE TO ARTHROMYCES RAMOSUS PEROXIDASE'''
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===BINDING MODE OF HYDROXYLAMINE TO ARTHROMYCES RAMOSUS PEROXIDASE===
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==Overview==
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The interaction of hydroxylamine (HA) with Arthromyces ramosus peroxidase (ARP) was investigated by kinetic, spectroscopic, and x-ray crystallographic techniques. HA inhibited the reaction of native ARP with H(2)O(2) in a competitive manner. Electron absorption and resonance Raman spectroscopic studies indicated that pentacoordinate high spin species of native ARP are converted to hexacoordinate low spin species upon the addition of HA, strongly suggesting the occurrence of a direct interaction of HA with ARP heme iron. Kinetic analysis exhibited that the apparent dissociation constant is 6.2 mm at pH 7.0 and that only one HA molecule likely binds to the vicinity of the heme. pH dependence of HA binding suggested that the nitrogen atom of HA could be involved in the interaction with the heme iron. X-ray crystallographic analysis of ARP in complex with HA at 2.0 A resolution revealed that the electron density ascribed to HA is located in the distal pocket between the heme iron and the distal His(56). HA seems to directly interact with the heme iron but is too far away to interact with Arg(52). In HA, it is likely that the nitrogen atom is coordinated to the heme iron and that hydroxyl group is hydrogen bonded to the distal His(56).
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(as it appears on PubMed at http://www.pubmed.gov), where 10915789 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10915789}}
==About this Structure==
==About this Structure==
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[[Category: Oxidoreductase]]
[[Category: Oxidoreductase]]
[[Category: Peroxidase]]
[[Category: Peroxidase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 12:27:36 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 20:21:54 2008''

Revision as of 17:21, 30 June 2008

Image:1c8i.png

Template:STRUCTURE 1c8i

BINDING MODE OF HYDROXYLAMINE TO ARTHROMYCES RAMOSUS PEROXIDASE

Template:ABSTRACT PUBMED 10915789

About this Structure

1C8I is a Single protein structure of sequence from Eukaryota. Full crystallographic information is available from OCA.

Reference

Direct binding of hydroxylamine to the heme iron of Arthromyces ramosus peroxidase. Substrate analogue that inhibits compound I formation in a competetive manner., Wariishi H, Nonaka D, Johjima T, Nakamura N, Naruta Y, Kubo S, Fukuyama K, J Biol Chem. 2000 Oct 20;275(42):32919-24. PMID:10915789

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