1eo0

From Proteopedia

Revision as of 12:02, 20 November 2007

CONSERVED DOMAIN COMMON TO TRANSCRIPTION FACTORS TFIIS, ELONGIN A, CRSP70

Overview

TFIIS is a transcription elongation factor that consists of three domains., We have previously solved the structures of domains II and III, which, stimulate arrested polymerase II elongation complexes in order to resume, transcription. Domain I is conserved in evolution from yeast to human, species and is homologous to the transcription factors elongin A and, CRSP70. Domain I also interacts with the transcriptionally active RNA, polymerase II holoenzyme and therefore, may have a function unrelated to, the previously described transcription elongation activity of TFIIS. We, have solved the structure of domain I of yeast TFIIS using NMR, spectroscopy. Domain I is a compact four-helix bundle that is structurally, independent of domains II and III of the TFIIS. Using the yeast structure, as a template, we have modeled the homologous domains from elongin A and, CRSP70 and identified a conserved positively charged patch on the surface, of all three proteins, which may be involved in conserved functional, interactions with the transcriptional machinery.

About this Structure

1EO0 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Structure of a conserved domain common to the transcription factors TFIIS, elongin A, and CRSP70., Booth V, Koth CM, Edwards AM, Arrowsmith CH, J Biol Chem. 2000 Oct 6;275(40):31266-8. PMID:10811649

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