1cee

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[[Image:1cee.gif|left|200px]]
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{{STRUCTURE_1cee| PDB=1cee | SCENE= }}
{{STRUCTURE_1cee| PDB=1cee | SCENE= }}
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'''SOLUTION STRUCTURE OF CDC42 IN COMPLEX WITH THE GTPASE BINDING DOMAIN OF WASP'''
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===SOLUTION STRUCTURE OF CDC42 IN COMPLEX WITH THE GTPASE BINDING DOMAIN OF WASP===
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==Overview==
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The Rho-family GTP-hydrolysing proteins (GTPases), Cdc42, Rac and Rho, act as molecular switches in signalling pathways that regulate cytoskeletal architecture, gene expression and progression of the cell cycle. Cdc42 and Rac transmit many signals through GTP-dependent binding to effector proteins containing a Cdc42/Rac-interactive-binding (CRIB) motif. One such effector, the Wiskott-Aldrich syndrome protein (WASP), is postulated to link activation of Cdc42 directly to the rearrangement of actin. Human mutations in WASP cause severe defects in haematopoletic cell function, leading to clinical symptoms of thrombocytopenia, immunodeficiency and eczema. Here we report the solution structure of a complex between activated Cdc42 and a minimal GTPase-binding domain (GBD) from WASP. An extended amino-terminal GBD peptide that includes the CRIB motif contacts the switch I, beta2 and alpha5 regions of Cdc42. A carboxy-terminal beta-hairpin and alpha-helix pack against switch II. The Phe-X-His-X2-His portion of the CRIB motif and the alpha-helix appear to mediate sensitivity to the nucleotide switch through contacts to residues 36-40 of Cdc42. Discrimination between the Rho-family members is likely to be governed by GBD contacts to the switch I and alpha5 regions of the GTPases. Structural and biochemical data suggest that GBD-sequence divergence outside the CRIB motif may reflect additional regulatory interactions with functional domains that are specific to individual effectors.
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(as it appears on PubMed at http://www.pubmed.gov), where 10360578 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10360578}}
==About this Structure==
==About this Structure==
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1CEE is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CEE OCA].
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1CEE is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CEE OCA].
==Reference==
==Reference==
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[[Category: Rosen, M K.]]
[[Category: Rosen, M K.]]
[[Category: Cdc42 actin regulator gtpase and the gtpase binding domain of its effector wasp]]
[[Category: Cdc42 actin regulator gtpase and the gtpase binding domain of its effector wasp]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 12:38:23 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 20:37:58 2008''

Revision as of 17:38, 30 June 2008

Image:1cee.png

Template:STRUCTURE 1cee

SOLUTION STRUCTURE OF CDC42 IN COMPLEX WITH THE GTPASE BINDING DOMAIN OF WASP

Template:ABSTRACT PUBMED 10360578

About this Structure

1CEE is a Protein complex structure of sequences from Homo sapiens. Full experimental information is available from OCA.

Reference

Structure of Cdc42 in complex with the GTPase-binding domain of the 'Wiskott-Aldrich syndrome' protein., Abdul-Manan N, Aghazadeh B, Liu GA, Majumdar A, Ouerfelli O, Siminovitch KA, Rosen MK, Nature. 1999 May 27;399(6734):379-83. PMID:10360578

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