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1epb
From Proteopedia
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(New page: 200px<br /><applet load="1epb" size="450" color="white" frame="true" align="right" spinBox="true" caption="1epb, resolution 2.2Å" /> '''STRUCTURE OF THE EPID...)
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Revision as of 12:04, 20 November 2007
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STRUCTURE OF THE EPIDIDYMAL RETINOIC ACID-BINDING PROTEIN AT 2.1 ANGSTROMS RESOLUTION
Overview
BACKGROUND: Androgen-dependent proteins in the lumen of the epididymis are, required for sperm maturation. One of these is a retinoic acid binding, protein, E-RABP, which binds both all-trans and 9-cis retinoic acid. The, other retinoid-binding proteins whose structures are known do not bind, 9-cis retinoids. RESULTS: We describe the X-ray structure determination of, E-RABP with and without bound ligand. The ligand binds deep in the, beta-barrel of the protein, the beta-ionone ring innermost. The binding, site, like the ligand, is amphipathic and the deepest part of the cavity, is formed by a ring of aromatic amino acids. The isoprene tail of, all-trans retinoic acid is bound in a folded conformation which resembles, that of the 9-cis isomer. CONCLUSION: E-RABP achieves high-affinity, binding of both all-trans and 9-cis isomers of retinoic acid by forcing, the all-trans form to bind in a folded conformation. The RAR family of, nuclear receptors for retinoic acid also binds both isomers, and their, binding sites may therefore be similar.
About this Structure
1EPB is a Single protein structure of sequence from Rattus norvegicus with REA as ligand. Full crystallographic information is available from OCA.
Reference
Structure of the epididymal retinoic acid binding protein at 2.1 A resolution., Newcomer ME, Structure. 1993 Sep 15;1(1):7-18. PMID:8069623
Page seeded by OCA on Tue Nov 20 14:11:57 2007
