1ch0

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1ch0.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:1ch0.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1ch0| PDB=1ch0 | SCENE= }}
{{STRUCTURE_1ch0| PDB=1ch0 | SCENE= }}
-
'''RNASE T1 VARIANT WITH ALTERED GUANINE BINDING SEGMENT'''
+
===RNASE T1 VARIANT WITH ALTERED GUANINE BINDING SEGMENT===
-
==Overview==
+
<!--
-
The ribonuclease T1 variant 9/5 with a guanine recognition segment, altered from the wild-type amino acid sequence 41-KYNNYE-46 to 41-EFRNWQ-46, has been cocrystallised with the specific inhibitor 2'-GMP. The crystal structure has been refined to a crystallographic R factor of 0.198 at 2.3 A resolution. Despite a size reduction of the binding pocket, pushing the inhibitor outside by 1 A, 2'-GMP is fixed to the primary recognition site due to increased aromatic stacking interactions. The phosphate group of 2'-GMP is located about 4.2 A apart from its position in wild-type ribonuclease T1-2'-GMP complexes, allowing a Ca(2+), coordinating this phosphate group, to enter the binding pocket. The crystallographic data can be aligned with the kinetic characterisation of the variant, showing a reduction of both, guanine affinity and turnover rate. The presence of Ca(2+) was shown to inhibit variant 9/5 and wild-type enzyme to nearly the same extent.
+
The line below this paragraph, {{ABSTRACT_PUBMED_10600381}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 10600381 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_10600381}}
==About this Structure==
==About this Structure==
Line 29: Line 33:
[[Category: Saenger, W.]]
[[Category: Saenger, W.]]
[[Category: Ribonuclease]]
[[Category: Ribonuclease]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 12:43:30 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 20:44:19 2008''

Revision as of 17:44, 30 June 2008

Image:1ch0.png

Template:STRUCTURE 1ch0

RNASE T1 VARIANT WITH ALTERED GUANINE BINDING SEGMENT

Template:ABSTRACT PUBMED 10600381

About this Structure

1CH0 is a Single protein structure of sequence from Aspergillus oryzae. Full crystallographic information is available from OCA.

Reference

Structural analysis of an RNase T1 variant with an altered guanine binding segment., Hoschler K, Hoier H, Hubner B, Saenger W, Orth P, Hahn U, J Mol Biol. 1999 Dec 17;294(5):1231-8. PMID:10600381

Page seeded by OCA on Mon Jun 30 20:44:19 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ch0"
Personal tools