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1cl0

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[[Image:1cl0.gif|left|200px]]
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{{STRUCTURE_1cl0| PDB=1cl0 | SCENE= }}
{{STRUCTURE_1cl0| PDB=1cl0 | SCENE= }}
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'''CRYSTAL STRUCTURE OF REDUCED THIOREDOXIN REDUCTASE FROM ESCHERICHIA COLI.'''
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===CRYSTAL STRUCTURE OF REDUCED THIOREDOXIN REDUCTASE FROM ESCHERICHIA COLI.===
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==Overview==
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Catalysis by thioredoxin reductase (TrxR) from Escherichia coli requires alternation between two domain arrangements. One of these conformations has been observed by X-ray crystallography (Waksman G, Krishna TSR, Williams CH Jr, Kuriyan J, 1994, J Mol Biol 236:800-816). This form of TrxR, denoted FO, permits the reaction of enzyme-bound reduced FAD with a redox-active disulfide on TrxR. As part of an investigation of conformational changes and intermediates in catalysis by TrxR, an X-ray structure of the FO form of TrxR with both the FAD and active site disulfide reduced has been determined. Reduction after crystallization resulted in significant local conformation changes. The isoalloxazine ring of the FAD cofactor, which is essentially planar in the oxidized enzyme, assumes a 34 degree "butterfly" bend about the N(5)-N(10) axis in reduced TrxR. Theoretical calculations reported by others predict ring bending of 15-28 degrees for reduced isoalloxazines protonated at N(1). The large bending in reduced TrxR is attributed in part to steric interactions between the isoalloxazine ring and the sulfur of Cys138, formed by reduction of the active site disulfide, and is accompanied by changes in the positions and interactions of several of the ribityl side-chain atoms of FAD. The bending angle in reduced TrxR is larger than that for any flavoprotein in the Protein Data Bank. Distributions of bending angles in published oxidized and reduced flavoenzyme structures are different from those found in studies of free flavins, indicating that the protein environment has a significant effect on bending.
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(as it appears on PubMed at http://www.pubmed.gov), where 10595539 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10595539}}
==About this Structure==
==About this Structure==
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[[Category: Ludwig, M L.]]
[[Category: Ludwig, M L.]]
[[Category: Flavoenzyme]]
[[Category: Flavoenzyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 12:50:59 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 20:53:44 2008''

Revision as of 17:53, 30 June 2008

Image:1cl0.png

Template:STRUCTURE 1cl0

CRYSTAL STRUCTURE OF REDUCED THIOREDOXIN REDUCTASE FROM ESCHERICHIA COLI.

Template:ABSTRACT PUBMED 10595539

About this Structure

1CL0 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystal structure of reduced thioredoxin reductase from Escherichia coli: structural flexibility in the isoalloxazine ring of the flavin adenine dinucleotide cofactor., Lennon BW, Williams CH Jr, Ludwig ML, Protein Sci. 1999 Nov;8(11):2366-79. PMID:10595539

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