1cow

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{{STRUCTURE_1cow| PDB=1cow | SCENE= }}
{{STRUCTURE_1cow| PDB=1cow | SCENE= }}
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'''BOVINE MITOCHONDRIAL F1-ATPASE COMPLEXED WITH AUROVERTIN B'''
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===BOVINE MITOCHONDRIAL F1-ATPASE COMPLEXED WITH AUROVERTIN B===
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==Overview==
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In the structure of bovine mitochondrial F1-ATPase that was previously determined with crystals grown in the presence of adenylyl-imidodiphosphate (AMP-PNP) and ADP, the three catalytic beta-subunits have different conformations and nucleotide occupancies. Adenylyl-imidodiphosphate is bound to one beta-subunit (betaTP), ADP is bound to the second (betaDP), and no nucleotide is bound to the third (betaE). Here we show that the uncompetitive inhibitor aurovertin B binds to bovine F1 at two equivalent sites in betaTP and betaE, in a cleft between the nucleotide binding and C-terminal domains. In betaDP, the aurovertin B pocket is incomplete and is inaccessible to the inhibitor. The aurovertin B bound to betaTP interacts with alpha-Glu399 in the adjacent alphaTP subunit, whereas the aurovertin B bound to betaE is too distant from alphaE to make an equivalent interaction. Both sites encompass betaArg-412, which was shown by mutational studies to be involved in binding aurovertin. Except for minor changes around the aurovertin pockets, the structure of bovine F1-ATPase is the same as determined previously. Aurovertin B appears to act by preventing closure of the catalytic interfaces, which is essential for a catalytic mechanism involving cyclic interconversion of catalytic sites.
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{{ABSTRACT_PUBMED_8692918}}
==About this Structure==
==About this Structure==
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[[Category: F1f atp synthase]]
[[Category: F1f atp synthase]]
[[Category: Hydrogen ion transport]]
[[Category: Hydrogen ion transport]]
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Revision as of 18:02, 30 June 2008

Image:1cow.png

Template:STRUCTURE 1cow

BOVINE MITOCHONDRIAL F1-ATPASE COMPLEXED WITH AUROVERTIN B

Template:ABSTRACT PUBMED 8692918

About this Structure

1COW is a Protein complex structure of sequences from Bos taurus. Full crystallographic information is available from OCA.

Reference

The structure of bovine F1-ATPase complexed with the antibiotic inhibitor aurovertin B., van Raaij MJ, Abrahams JP, Leslie AG, Walker JE, Proc Natl Acad Sci U S A. 1996 Jul 9;93(14):6913-7. PMID:8692918

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