1cpm

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{{STRUCTURE_1cpm| PDB=1cpm | SCENE= }}
{{STRUCTURE_1cpm| PDB=1cpm | SCENE= }}
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'''NATIVE-LIKE IN VIVO FOLDING OF A CIRCULARLY PERMUTED JELLYROLL PROTEIN SHOWN BY CRYSTAL STRUCTURE ANALYSIS'''
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===NATIVE-LIKE IN VIVO FOLDING OF A CIRCULARLY PERMUTED JELLYROLL PROTEIN SHOWN BY CRYSTAL STRUCTURE ANALYSIS===
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==Overview==
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A jellyroll beta-sandwich protein, the Bacillus beta-glucanase H(A16-M), is used to probe the role of N-terminal peptide regions in protein folding in vivo. A gene encoding H(A16-M) is rearranged to place residues 1-58 of the protein behind a signal peptide and residues 59-214. The rearranged gene is expressed in Escherichia coli. The resultant circularly permuted protein, cpA16M-59, is secreted into the periplasm, correctly processed, and folded into a stable and active enzyme. Crystal structure analysis at 2.0-A resolution, R = 15.3%, shows cpA16M-59 to have a three-dimensional structure nearly identical with that of the parent beta-glucanase. An analogous experiment based on the wild-type Bacillus macerans beta-glucanase, giving rise to the circularly permuted variant cpMAC-57, yields the same results. Folding of these proteins, therefore, is not a vectorial process depending on the conformation adopted by their native N-terminal oligopeptides after ribosomal synthesis and translocation through the cytoplasmic membrane.
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(as it appears on PubMed at http://www.pubmed.gov), where 7937966 is the PubMed ID number.
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{{ABSTRACT_PUBMED_7937966}}
==About this Structure==
==About this Structure==
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[[Category: Hahn, M.]]
[[Category: Hahn, M.]]
[[Category: Heinemann, U.]]
[[Category: Heinemann, U.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 12:58:45 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 21:03:54 2008''

Revision as of 18:03, 30 June 2008

Image:1cpm.png

Template:STRUCTURE 1cpm

NATIVE-LIKE IN VIVO FOLDING OF A CIRCULARLY PERMUTED JELLYROLL PROTEIN SHOWN BY CRYSTAL STRUCTURE ANALYSIS

Template:ABSTRACT PUBMED 7937966

About this Structure

1CPM is a Single protein structure of sequence from Paenibacillus macerans. Full crystallographic information is available from OCA.

Reference

Native-like in vivo folding of a circularly permuted jellyroll protein shown by crystal structure analysis., Hahn M, Piotukh K, Borriss R, Heinemann U, Proc Natl Acad Sci U S A. 1994 Oct 25;91(22):10417-21. PMID:7937966

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