1cw9

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[[Image:1cw9.gif|left|200px]]
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{{Seed}}
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[[Image:1cw9.png|left|200px]]
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{{STRUCTURE_1cw9| PDB=1cw9 | SCENE= }}
{{STRUCTURE_1cw9| PDB=1cw9 | SCENE= }}
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'''DNA DECAMER WITH AN ENGINEERED CROSSLINK IN THE MINOR GROOVE'''
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===DNA DECAMER WITH AN ENGINEERED CROSSLINK IN THE MINOR GROOVE===
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==Overview==
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The response of double-helical DNA to torsional stress may be a driving force for many processes acting on DNA. The 1.55-A crystal structure of a duplex DNA oligonucleotide d(CCAGGCCTGG)(2) with an engineered crosslink in the minor groove between the central guanine bases depicts how the duplex can accommodate such torsional stress. We have captured in the same crystal two rather different conformational states. One duplex contains a strained crosslink that is stabilized by calcium ion binding in the major groove, directly opposite the crosslink. For the other duplex, the strain in the crosslink is relieved through partial rupture of a base pair and partial extrusion of a cytosine accompanied by helix bending. The sequence used is the target sequence for the HaeIII methylase, and this partially flipped cytosine is the same nucleotide targeted for extrusion by the enzyme. Molecular dynamics simulations of these structures show an increased mobility for the partially flipped-out cytosine.
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The line below this paragraph, {{ABSTRACT_PUBMED_10518532}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 10518532 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10518532}}
==About this Structure==
==About this Structure==
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[[Category: Crosslinked double-helical dna]]
[[Category: Crosslinked double-helical dna]]
[[Category: Partial base flipping.]]
[[Category: Partial base flipping.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 13:10:54 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 21:36:13 2008''

Revision as of 18:36, 30 June 2008

Image:1cw9.png

Template:STRUCTURE 1cw9

DNA DECAMER WITH AN ENGINEERED CROSSLINK IN THE MINOR GROOVE

Template:ABSTRACT PUBMED 10518532

About this Structure

Full crystallographic information is available from OCA.

Reference

A structural snapshot of base-pair opening in DNA., van Aalten DM, Erlanson DA, Verdine GL, Joshua-Tor L, Proc Natl Acad Sci U S A. 1999 Oct 12;96(21):11809-14. PMID:10518532

Page seeded by OCA on Mon Jun 30 21:36:13 2008

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