1cx7

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[[Image:1cx7.gif|left|200px]]
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{{STRUCTURE_1cx7| PDB=1cx7 | SCENE= }}
{{STRUCTURE_1cx7| PDB=1cx7 | SCENE= }}
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'''T4 LYSOZYME METHIONINE CORE MUTANT'''
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===T4 LYSOZYME METHIONINE CORE MUTANT===
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==Overview==
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Using heavily methionine-substituted T4 lysozyme as an example, it is shown how the addition or deletion of a small number of methionines can simplify the location of selenium sites for use in MAD phasing. By comparing the X-ray data for a large number of singly substituted lysozymes, it is shown that the optimal amino acid to be substituted by methionine is leucine, followed, in order of preference, by phenylalanine, isoleucine and valine. The identification of leucine as the first choice agrees with the ranking suggested by the Dayhoff mutation probability, i.e. by the frequency of amino-acid substitutions in the sequences of related proteins. The ranking of the second and subsequent choices, however, differ significantly.
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(as it appears on PubMed at http://www.pubmed.gov), where 10666571 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10666571}}
==About this Structure==
==About this Structure==
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[[Category: Protein folding]]
[[Category: Protein folding]]
[[Category: T4 lysozyme]]
[[Category: T4 lysozyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 13:12:44 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 21:42:04 2008''

Revision as of 18:42, 30 June 2008

Image:1cx7.png

Template:STRUCTURE 1cx7

T4 LYSOZYME METHIONINE CORE MUTANT

Template:ABSTRACT PUBMED 10666571

About this Structure

1CX7 is a Single protein structure of sequence from Enterobacteria phage t4. Full crystallographic information is available from OCA.

Reference

Use of differentially substituted selenomethionine proteins in X-ray structure determination., Gassner NC, Matthews BW, Acta Crystallogr D Biol Crystallogr. 1999 Dec;55(Pt 12):1967-70. PMID:10666571

Page seeded by OCA on Mon Jun 30 21:42:04 2008

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