1d29

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{{STRUCTURE_1d29| PDB=1d29 | SCENE= }}
{{STRUCTURE_1d29| PDB=1d29 | SCENE= }}
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'''CRYSTAL STRUCTURE ANALYSIS OF THE B-DNA DODECAMER CGTGAATTCACG'''
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===CRYSTAL STRUCTURE ANALYSIS OF THE B-DNA DODECAMER CGTGAATTCACG===
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==Overview==
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The crystal structure of the DNA dodecamer C-G-T-G-A-A-T-T-C-A-C-G has been determined at a resolution of 2.5 A, with a final R factor of 15.8% for 1475 nonzero reflections measured at 0 degrees C. The structure is isomorphous with that of the Drew dodecamer, with the space group P2(1)2(1)2(1) and cell dimensions of a = 24.94 A, b = 40.78 A, and c = 66.13 A. The asymmetric unit contains all 12 base pairs of the B-DNA double helix and 36 water molecules. The structure of C-G-T-G-A-A-T-T-C-A-C-G is very similar to that of C-G-C-G-A-A-T-T-C-G-C-G, with no major alterations in helix parameters. Water peaks in the refined structure appear to represent a selection of peaks that were observed in the Drew dodecamer. The minor-groove spine of hydration at 2.5 A is fragmentary, but as Narendra et al. (1991) [Biochemistry (following paper in this issue)] have observed, lowering the temperature leads to a more complete representation of the spine.
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(as it appears on PubMed at http://www.pubmed.gov), where 1850624 is the PubMed ID number.
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{{ABSTRACT_PUBMED_1850624}}
==About this Structure==
==About this Structure==
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[[Category: B-dna]]
[[Category: B-dna]]
[[Category: Double helix]]
[[Category: Double helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 13:22:02 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 22:10:58 2008''

Revision as of 19:11, 30 June 2008

Image:1d29.png

Template:STRUCTURE 1d29

CRYSTAL STRUCTURE ANALYSIS OF THE B-DNA DODECAMER CGTGAATTCACG

Template:ABSTRACT PUBMED 1850624

About this Structure

Full crystallographic information is available from OCA.

Reference

Crystal structure analysis of the B-DNA dodecamer CGTGAATTCACG., Larsen TA, Kopka ML, Dickerson RE, Biochemistry. 1991 May 7;30(18):4443-9. PMID:1850624

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