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| - | [[Image:1d4z.jpg|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1d4z| PDB=1d4z | SCENE= }} | | {{STRUCTURE_1d4z| PDB=1d4z | SCENE= }} |
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| - | '''CRYSTAL STRUCTURE OF CHEY-95IV, A HYPERACTIVE CHEY MUTANT'''
| + | ===CRYSTAL STRUCTURE OF CHEY-95IV, A HYPERACTIVE CHEY MUTANT=== |
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| - | ==Overview==
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| - | In Escherichia coli, swimming behavior is mediated by the phosphorylation state of the response regulator CheY. In its active, phosphorylated form, CheY exhibits enhanced binding to a switch component, FliM, at the flagellar motor, which induces a change from counterclockwise to clockwise flagellar rotation. When Ile(95) of CheY is replaced by a valine, increased clockwise rotation correlates with enhanced binding to FliM. A possible explanation for the hyperactivity of this mutant is that residue 95 affects the conformation of nearby residues that potentially interact with FliM. In order to assess this possibility directly, the crystal structure of CheY95IV was determined. We found that CheY95IV is structurally almost indistinguishable from wild-type CheY. Several other mutants with substitutions at position 95 were characterized to establish the structural requirements for switch binding and clockwise signaling at this position and to investigate a general relationship between the two properties. The various rotational phenotypes of these mutants can be explained solely by the amount of phosphorylated CheY bound to the switch, which was inferred from the phosphorylation properties of the mutant CheY proteins and their binding affinities to FliM. Combined genetic, biochemical, and crystallographic results suggest that residue 95 itself is critical in mediating the surface complementarity between CheY and FliM.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_10748173}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 10748173 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_10748173}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Response regulator]] | | [[Category: Response regulator]] |
| | [[Category: Signaling protein]] | | [[Category: Signaling protein]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 13:27:40 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 22:25:57 2008'' |
Revision as of 19:25, 30 June 2008
Template:STRUCTURE 1d4z
CRYSTAL STRUCTURE OF CHEY-95IV, A HYPERACTIVE CHEY MUTANT
Template:ABSTRACT PUBMED 10748173
About this Structure
1D4Z is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Correlated switch binding and signaling in bacterial chemotaxis., Schuster M, Zhao R, Bourret RB, Collins EJ, J Biol Chem. 2000 Jun 30;275(26):19752-8. PMID:10748173
Page seeded by OCA on Mon Jun 30 22:25:57 2008
