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| | {{STRUCTURE_1d6j| PDB=1d6j | SCENE= }} | | {{STRUCTURE_1d6j| PDB=1d6j | SCENE= }} |
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| - | '''CRYSTAL STRUCTURE OF ADENOSINE 5'-PHOSPHOSULFATE (APS) KINASE FROM PENICILLIUM CHRYSOGENUM'''
| + | ===CRYSTAL STRUCTURE OF ADENOSINE 5'-PHOSPHOSULFATE (APS) KINASE FROM PENICILLIUM CHRYSOGENUM=== |
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| - | ==Overview==
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| - | Adenosine 5'-phosphosulfate (APS) kinase catalyzes the second reaction in the two-step conversion of inorganic sulfate to 3'-phosphoadenosine 5'-phosphosulfate (PAPS). This report presents the 2.0 A resolution crystal structure of ligand-free APS kinase from the filamentous fungus, Penicillium chrysogenum. The enzyme crystallized as a homodimer with each subunit folded into a classic kinase motif consisting of a twisted, parallel beta-sheet sandwiched between two alpha-helical bundles. The Walker A motif, (32)GLSASGKS(39), formed the predicted P-loop structure. Superposition of the APS kinase active site region onto several other P-loop-containing proteins revealed that the conserved aspartate residue that usually interacts with the Mg(2+) coordination sphere of MgATP is absent in APS kinase. However, upon MgATP binding, a different aspartate, Asp 61, could shift and bind to the Mg(2+). The sequence (156)KAREGVIKEFT(166), which has been suggested to be a (P)APS motif, is located in a highly protease-susceptible loop that is disordered in both subunits of the free enzyme. MgATP or MgADP protects against proteolysis; APS alone has no effect but augments the protection provided by MgADP. The results suggest that the loop lacks a fixed structure until MgATP or MgADP is bound. The subsequent conformational change together with the potential change promoted by the interaction of MgATP with Asp 61 may define the APS binding site. This model is consistent with the obligatory ordered substrate binding sequence (MgATP or MgADP before APS) as established from steady state kinetics and equilibrium binding studies.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_10677210}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 10677210 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_10677210}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Sulfate]] | | [[Category: Sulfate]] |
| | [[Category: Transferase]] | | [[Category: Transferase]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 13:30:22 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 22:32:23 2008'' |
Revision as of 19:32, 30 June 2008
Template:STRUCTURE 1d6j
CRYSTAL STRUCTURE OF ADENOSINE 5'-PHOSPHOSULFATE (APS) KINASE FROM PENICILLIUM CHRYSOGENUM
Template:ABSTRACT PUBMED 10677210
About this Structure
1D6J is a Single protein structure of sequence from Penicillium chrysogenum. Full crystallographic information is available from OCA.
Reference
Crystal structure of adenosine 5'-phosphosulfate kinase from Penicillium chrysogenum., MacRae IJ, Segel IH, Fisher AJ, Biochemistry. 2000 Feb 22;39(7):1613-21. PMID:10677210
Page seeded by OCA on Mon Jun 30 22:32:23 2008
